2iy8: Difference between revisions

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caption="2iy8, resolution 2.5Å" />

'''CRYSTAL STRUCTURE OF THE SIALYLTRANSFERASE PM0188 WITH CMP-3FNEUAC AND LACTOSE'''<br />

==Overview==

PM0188 is a newly identified sialyltransferase from P. multocida which transfers sialic acid from cytidine 5'-monophosphonuraminic acid (CMP-NeuAc) to an acceptor sugar. Although sialyltransferases are involved in important biological functions like cell-cell recognition, cell differentiation and receptor-ligand interactions, little is known about their catalytic mechanism. Here, we report the X-ray crystal structures of PM0188 in the presence of an acceptor sugar and a donor sugar analogue, revealing the precise mechanism of sialic acid transfer. Site-directed mutagenesis, kinetic assays, and structural analysis show that Asp141, His311, Glu338, Ser355 and Ser356 are important catalytic residues; Asp141 is especially crucial as it acts as a general base. These complex structures provide insights into the mechanism of sialyltransferases and the structure-based design of specific inhibitors.

==About this Structure==

2IY8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pasteurella_multocida Pasteurella multocida] with <scene name='pdbligand=LAT:'>LAT</scene> and <scene name='pdbligand=CSF:'>CSF</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional ~~Site~~: <scene name='pdbsite=AC1:Csf+Binding+Site+For+Chain+A'>~~AC1~~</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IY8 OCA].

2IY8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pasteurella_multocida Pasteurella multocida] with <scene name='pdbligand=LAT:'>LAT</scene> and <scene name='pdbligand=CSF:'>CSF</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=AC1:Lat+Binding+Site+For+Chain+A'>AC1</scene> and <scene name='pdbsite=AC2:Csf+Binding+Site+For+Chain+A'>AC2</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IY8 OCA].

==Reference==

Structural analysis of sialyltransferase PM0188 from Pasteurella multocida complexed with donor analogue and acceptor sugar., Kim DU, Yoo JH, Lee YJ, Kim KS, Cho HS, BMB Rep. 2008 Jan 31;41(1):48-54. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18304450 18304450]

[[Category: Pasteurella multocida]]

[[Category: Single protein]]

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[[Category: transferase]]

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@@ Line 2: / Line 2: @@
 caption="2iy8, resolution 2.5&Aring;" />
 '''CRYSTAL STRUCTURE OF THE SIALYLTRANSFERASE PM0188 WITH CMP-3FNEUAC AND LACTOSE'''<br />
+==Overview==
+PM0188 is a newly identified sialyltransferase from P. multocida which transfers sialic acid from cytidine 5'-monophosphonuraminic acid (CMP-NeuAc) to an acceptor sugar. Although sialyltransferases are involved in important biological functions like cell-cell recognition, cell differentiation and receptor-ligand interactions, little is known about their catalytic mechanism. Here, we report the X-ray crystal structures of PM0188 in the presence of an acceptor sugar and a donor sugar analogue, revealing the precise mechanism of sialic acid transfer. Site-directed mutagenesis, kinetic assays, and structural analysis show that Asp141, His311, Glu338, Ser355 and Ser356 are important catalytic residues; Asp141 is especially crucial as it acts as a general base. These complex structures provide insights into the mechanism of sialyltransferases and the structure-based design of specific inhibitors.
 ==About this Structure==
-IY8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pasteurella_multocida Pasteurella multocida] with <scene name='pdbligand=LAT:'>LAT</scene> and <scene name='pdbligand=CSF:'>CSF</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Csf+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IY8 OCA].
+IY8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pasteurella_multocida Pasteurella multocida] with <scene name='pdbligand=LAT:'>LAT</scene> and <scene name='pdbligand=CSF:'>CSF</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=AC1:Lat+Binding+Site+For+Chain+A'>AC1</scene> and <scene name='pdbsite=AC2:Csf+Binding+Site+For+Chain+A'>AC2</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IY8 OCA].
+==Reference==
+Structural analysis of sialyltransferase PM0188 from Pasteurella multocida complexed with donor analogue and acceptor sugar., Kim DU, Yoo JH, Lee YJ, Kim KS, Cho HS, BMB Rep. 2008 Jan 31;41(1):48-54. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18304450 18304450]
 [[Category: Pasteurella multocida]]
 [[Category: Single protein]]
@@ Line 18: / Line 24: @@
 [[Category: transferase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:57:19 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Mar 14 09:42:39 2008''