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[[Image:3aml.jpg|left|200px]]
==Structure of the Starch Branching Enzyme I (BEI) from Oryza sativa L==
<StructureSection load='3aml' size='340' side='right' caption='[[3aml]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3aml]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Oryza_sativa_japonica_group Oryza sativa japonica group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AML OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AML FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SIN:SUCCINIC+ACID'>SIN</scene><br>
<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3amk|3amk]]</td></tr>
<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Os06g0726400, SBE1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=39947 Oryza sativa Japonica Group])</td></tr>
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3aml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aml OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3aml RCSB], [http://www.ebi.ac.uk/pdbsum/3aml PDBsum]</span></td></tr>
<table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Starch-branching enzyme catalyzes the cleavage of alpha-1, 4-linkages and the subsequent transfer of alpha-1,4 glucan to form an alpha-1,6 branch point in amylopectin. Sequence analysis of the rice-branching enzyme I (BEI) indicated a modular structure in which the central alpha-amylase domain is flanked on each side by the N-terminal carbohydrate-binding module 48 and the alpha-amylase C-domain. We determined the crystal structure of BEI at a resolution of 1.9 A by molecular replacement using the Escherichia coli glycogen BE as a search model. Despite three modular structures, BEI is roughly ellipsoidal in shape with two globular domains that form a prominent groove which is proposed to serve as the alpha-polyglucan-binding site. Amino acid residues Asp344 and Glu399, which are postulated to play an essential role in catalysis as a nucleophile and a general acid/base, respectively, are located at a central cleft in the groove. Moreover, structural comparison revealed that in BEI, extended loop structures cause a narrowing of the substrate-binding site, whereas shortened loop structures make a larger space at the corresponding subsite in the Klebsiella pneumoniae pullulanase. This structural difference might be attributed to distinct catalytic reactions, transglycosylation and hydrolysis, respectively, by BEI and pullulanase.


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Crystal structure of the branching enzyme I (BEI) from Oryza sativa L with implications for catalysis and substrate binding.,Noguchi J, Chaen K, Vu NT, Akasaka T, Shimada H, Nakashima T, Nishi A, Satoh H, Omori T, Kakuta Y, Kimura M Glycobiology. 2011 Aug;21(8):1108-16. Epub 2011 Apr 14. PMID:21493662<ref>PMID:21493662</ref>
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{{STRUCTURE_3aml|  PDB=3aml  |  SCENE=  }}


===Structure of the Starch Branching Enzyme I (BEI) from Oryza sativa L===
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
 
</div>
 
== References ==
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{{ABSTRACT_PUBMED_21493662}}
 
==About this Structure==
[[3aml]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Oryza_sativa_japonica_group Oryza sativa japonica group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AML OCA].
 
==Reference==
<ref group="xtra">PMID:021493662</ref><references group="xtra"/>
[[Category: Oryza sativa japonica group]]
[[Category: Oryza sativa japonica group]]
[[Category: Chaen, K.]]
[[Category: Chaen, K.]]

Revision as of 11:04, 14 May 2014

Structure of the Starch Branching Enzyme I (BEI) from Oryza sativa L

3aml, resolution 1.70Å

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