Talk:Resolution: Difference between revisions
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Proposed revision (May, 2014) after discussion with Keiichi Namba. Comments welcome! | Proposed revision (May, 2014) after discussion with Keiichi Namba. Comments welcome! | ||
Resolution of a macromolecular crystal is a value that quantitates the uncertainty of atomic positions in the portion of the macromolecule that is most ordered in the crystal. High numeric values (such as 4 Å) mean high uncertainty, "poor resolution"; low values (such as 1.5 Å) mean low uncertainty, "good resolution". The most-ordered portion of the macromolecule is responsible for diffraction spots that are farthest from the axis of the X-ray beam. | Resolution of a macromolecular crystal is a value that quantitates the uncertainty of atomic positions in the portion of the macromolecule that is most ordered in the crystal. High numeric values (such as 4 Å) mean high uncertainty, "poor resolution"; low values (such as 1.5 Å) mean low uncertainty, "good resolution". The most-ordered portion of the macromolecule is responsible for diffraction spots that are farthest from the axis of the X-ray beam. Resolution is determined from these farthest spots based on their angle θ from the X-ray beam, using [http://en.wikipedia.org/wiki/Bragg's_law the Bragg equation]: | ||
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d = λ/2sinθ | |||
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where λ is the X-ray wavelength, and ''d'' is the smallest distance between crystal lattice planes that scatter discrete spots. The resolution is given by ''d''. | |||
--[[User:Eric Martz|Eric Martz]] 13:17, 15 May 2014 (IDT) | --[[User:Eric Martz|Eric Martz]] 13:17, 15 May 2014 (IDT) | ||
