Sandbox Reserved 930: Difference between revisions
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==The subdomains of the motor domain== | ==The subdomains of the motor domain== | ||
The MD of S1 unit is most frequently described as consisting of 4 subdomains: the converter, the N-terminal subdomain, and upper and lower 50-kDa subdomains <ref>PMID: 10338210</ref>. They are linked together by 3 single-stranded joints termed the switch II (residue IIe-461 to Asn-470), the relay (residues Asn-489 to ASP-519), and SH1 helix (Cys-693 to Phe-707) <ref>PMID: 11016966</ref> | The MD of S1 unit is most frequently described as consisting of 4 subdomains: the converter, the N-terminal subdomain, and upper and lower 50-kDa subdomains <ref>PMID: 10338210</ref>. They are linked together by 3 single-stranded joints termed the switch II (residue IIe-461 to Asn-470), the relay (residues Asn-489 to ASP-519), and SH1 helix (Cys-693 to Phe-707)(Fig. 3) <ref>PMID: 11016966</ref>. | ||
• Of the MD subdomains the converter has the greatest positional change during contractile cycle. Connection of the converter and the lever arm allows relatively small changes in the converter to be greatly amplified in the lever arm | • Of the MD subdomains the converter has the greatest positional change during contractile cycle. Connection of the converter and the lever arm allows relatively small changes in the converter to be greatly amplified in the lever arm | ||
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• Upper 50-kDa subdomain and N-terminal subdomain form the nucleotide binding pocket | • Upper 50-kDa subdomain and N-terminal subdomain form the nucleotide binding pocket | ||
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• Lower and upper 50-kDa subdomains form the interface where actin can bind<ref>PMID: 11016966</ref> | • Lower and upper 50-kDa subdomains form the interface where actin can bind <ref>PMID: 11016966</ref> | ||
Conformational changes in the flexible joints coordinate rearrangements of these four MD subdomains enabling the transition between different myosin S1 unit state within actomyosin contractile cycle. During the actomyosin cycle the MD undergoes many conformational changes as it traduces ATP hydrolysis to mechanical work. The different conformational states of myosin are termed strong or weak actin-binding states <ref>PMID: 15184651</ref>. | Conformational changes in the flexible joints coordinate rearrangements of these four MD subdomains enabling the transition between different myosin S1 unit state within actomyosin contractile cycle. During the actomyosin cycle the MD undergoes many conformational changes as it traduces ATP hydrolysis to mechanical work. The different conformational states of myosin are termed strong or weak actin-binding states <ref>PMID: 15184651</ref>. | ||
