Sandbox Reserved 930: Difference between revisions
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Myosin is a large asymmetric molecule with a MW of about 500,000 kDa. It consist of two globular head domains termed myosin subfragment 1 (S1), one neck subfragment 2 (S2) and a light meromyosin tail (LMM) <ref>PMID: 8203020</ref>. Myosin S1 unit comprises of a motor domain (MD) and a lever arm (Fig.3). By 2000 the structures of three scallop myosin S1 isoforms have been determined <ref>PMID: 11016966</ref><ref>PMID: 10338210</ref>, which are: | Myosin is a large asymmetric molecule with a MW of about 500,000 kDa. It consist of two globular head domains termed myosin subfragment 1 (S1), one neck subfragment 2 (S2) and a light meromyosin tail (LMM) <ref>PMID: 8203020</ref>. Myosin S1 unit comprises of a motor domain (MD) and a lever arm (Fig.3). By 2000 the structures of three scallop myosin S1 isoforms have been determined <ref>PMID: 11016966</ref><ref>PMID: 10338210</ref>, which are: | ||
• S1 nucleotide-free state corresponding to the rigor state of myosin, actin complex | • S1 nucleotide-free state corresponding to the rigor state of myosin, actin complex [http://www.pdb.org/pdb/explore/explore.do?structureId=1DFK 1DFK] | ||
• S1 Mg-ADP.VO4 state corresponding to the transition state (1kk8) | • S1 Mg-ADP.VO4 state corresponding to the transition state (1kk8) | ||
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==The subdomains of the motor domain== | ==The subdomains of the motor domain== | ||
[[Image:SUBDOMAINS.gif|300px|right|thumb|Figure 3. The | [[Image:SUBDOMAINS.gif|300px|right|thumb|Figure 3. The subdomains on myosin S1 unit] | ||
The MD of S1 unit is most frequently described as consisting of 4 subdomains: the converter, the N-terminal subdomain, and upper and lower 50-kDa subdomains <ref>PMID: 10338210</ref>. They are linked together by 3 single-stranded joints termed the switch II (residue IIe-461 to Asn-470), the relay (residues Asn-489 to ASP-519), and SH1 helix (Cys-693 to Phe-707)(Fig. 3) <ref>PMID: 11016966</ref>. | The MD of S1 unit is most frequently described as consisting of 4 subdomains: the converter, the N-terminal subdomain, and upper and lower 50-kDa subdomains <ref>PMID: 10338210</ref>. They are linked together by 3 single-stranded joints termed the switch II (residue IIe-461 to Asn-470), the relay (residues Asn-489 to ASP-519), and SH1 helix (Cys-693 to Phe-707)(Fig. 3) <ref>PMID: 11016966</ref>. | ||
