Sandbox Reserved 930: Difference between revisions
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• S1 nucleotide-free state corresponding to the rigor state of myosin, actin complex [http://www.pdb.org/pdb/explore/explore.do?structureId=1DFK 1DFK] | • S1 nucleotide-free state corresponding to the rigor state of myosin, actin complex [http://www.pdb.org/pdb/explore/explore.do?structureId=1DFK 1DFK] | ||
• S1 Mg-ADP.VO4 state corresponding to the transition state | • S1 Mg-ADP.VO4 state corresponding to the transition state [http://www.pdb.org/pdb/explore/explore.do?structureId=1kk8 1KK8] | ||
• S1 Mg-ADP state corresponding to the myosin detached state | • S1 Mg-ADP state corresponding to the myosin detached state [http://www.rcsb.org/pdb/explore/explore.do?structureId=1b7t 1B7T] | ||
By comparing the available crystal structures of different myosin S1 unit isoforms, it enables us to understand the conformational changes within the motor domain depending on the nucleotide content in the active site. Here mainly the structure and function of MD relevant in the S1 Mg-ADP (pre power stroke) state will be discussed. | By comparing the available crystal structures of different myosin S1 unit isoforms, it enables us to understand the conformational changes within the motor domain depending on the nucleotide content in the active site. Here mainly the structure and function of MD relevant in the S1 Mg-ADP (pre power stroke) state will be discussed. | ||
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==Nucleotide binding pocket: ADP + | ==Nucleotide binding pocket: ADP + Mg<sup>2+</sup>== | ||
The nucleotide-binding pocket is located at the interface of the 50 kDa upper subdomain and the N-terminal subdomain, which is opposite a deep cleft that bisects the actin-binding domain (the domain picture).This part of protein involves an arrangement of a secondary structure mainly around the parallel 7-stranded <scene name='57/579700/Strands/1'> β-sheet</scene> (reference 1). Loops extending from b-strands interact with the adenine nucleotide. | The nucleotide-binding pocket is located at the interface of the 50 kDa upper subdomain and the N-terminal subdomain, which is opposite a deep cleft that bisects the actin-binding domain (the domain picture).This part of protein involves an arrangement of a secondary structure mainly around the parallel 7-stranded <scene name='57/579700/Strands/1'> β-sheet</scene> (reference 1). Loops extending from b-strands interact with the adenine nucleotide. | ||
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</StructureSection> | </StructureSection> | ||
==References== | |||
<references/> | |||
