Sandbox Reserved 930: Difference between revisions
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==The subdomains of the motor domain== | ==The subdomains of the motor domain== | ||
[[Image:SUBDOMAINS.gif|400px|right|thumb|Figure 3. The subdomains on myosin S1 unit (Houdusse 2000)]] | [[Image:SUBDOMAINS.gif|400px|right|thumb|Figure 3. The subdomains on myosin S1 unit (Houdusse 2000)]] | ||
The MD of the scallop S1 unit is most frequently described as consisting of four subdomains: the converter, the N-terminal subdomain, and the upper and lower 50-kDa subdomains <ref>PMID: 10338210</ref>. They are linked together by three single-stranded joints termed the switch II (residues IIe-461 to Asn-470), the relay (residues Asn-489 to Asp-519), and SH1 helix (residues Cys-693 to Phe-707) (Fig. 3) <ref>PMID: 11016966</ref>. | The MD of the scallop S1 unit is most frequently described as consisting of four subdomains: the converter, the N-terminal subdomain, and the upper and lower 50-kDa subdomains <ref>PMID: 10338210</ref>. They are linked together by three single-stranded joints termed the switch II (residues IIe-461 to Asn-470), the relay (residues Asn-489 to Asp-519), and SH1 helix (residues Cys-693 to Phe-707) (Fig. 3) <ref name=11016966>PMID: 11016966</ref>. | ||
• Of the MD subdomains the converter changes its position the most during the contractile cycle. Connection of the converter and the lever arm allows relatively small changes in the converter to be greatly amplified in the lever arm (Fig. 4) | • Of the MD subdomains the converter changes its position the most during the contractile cycle. Connection of the converter and the lever arm allows relatively small changes in the converter to be greatly amplified in the lever arm (Fig. 4) | ||
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• Upper 50-kDa subdomain and N-terminal subdomain form the nucleotide binding pocket | • Upper 50-kDa subdomain and N-terminal subdomain form the nucleotide binding pocket | ||
• Lower and upper 50-kDa subdomains form the interface where actin can bind <ref | • Lower and upper 50-kDa subdomains form the interface where actin can bind <ref name=11016966 /> | ||
Conformational changes in the flexible joints coordinate rearrangements of the four MD subdomains enabling the transition between different myosin S1 conformations in the actomyosin contractile cycle, during which S1 traduces ATP hydrolysis to mechanical work. The different conformational states of myosin are termed strong or weak actin-binding states <ref>PMID: 15184651</ref>. | Conformational changes in the flexible joints coordinate rearrangements of the four MD subdomains enabling the transition between different myosin S1 conformations in the actomyosin contractile cycle, during which S1 traduces ATP hydrolysis to mechanical work. The different conformational states of myosin are termed strong or weak actin-binding states <ref>PMID: 15184651</ref>. | ||
