1a65: Difference between revisions
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'''TYPE-2 CU-DEPLETED LACCASE FROM COPRINUS CINEREUS''' | {{Structure | ||
|PDB= 1a65 |SIZE=350|CAPTION= <scene name='initialview01'>1a65</scene>, resolution 2.23Å | |||
|SITE= <scene name='pdbsite=T1:Type+1+Cu+Binding+Site'>T1</scene> and <scene name='pdbsite=T3:Type+3+Cu+Binding+Site'>T3</scene> | |||
|LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=GLC:GLUCOSE'>GLC</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene> and <scene name='pdbligand=O:OXYGEN ATOM'>O</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Laccase Laccase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] | |||
|GENE= | |||
}} | |||
'''TYPE-2 CU-DEPLETED LACCASE FROM COPRINUS CINEREUS''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1A65 is a [ | 1A65 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Coprinopsis_cinerea Coprinopsis cinerea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A65 OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of the type-2 Cu depleted laccase from Coprinus cinereus at 2.2 A resolution., Ducros V, Brzozowski AM, Wilson KS, Brown SH, Ostergaard P, Schneider P, Yaver DS, Pedersen AH, Davies GJ, Nat Struct Biol. 1998 Apr;5(4):310-6. PMID:[http:// | Crystal structure of the type-2 Cu depleted laccase from Coprinus cinereus at 2.2 A resolution., Ducros V, Brzozowski AM, Wilson KS, Brown SH, Ostergaard P, Schneider P, Yaver DS, Pedersen AH, Davies GJ, Nat Struct Biol. 1998 Apr;5(4):310-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9546223 9546223] | ||
[[Category: Coprinopsis cinerea]] | [[Category: Coprinopsis cinerea]] | ||
[[Category: Laccase]] | [[Category: Laccase]] | ||
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[[Category: type-2 copper depleted]] | [[Category: type-2 copper depleted]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 09:53:30 2008'' | ||
Revision as of 07:53, 20 March 2008
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| 1a65, resolution 2.23Å | |||||||||||||
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| Sites: | T1 and T3 | ||||||||||||
| Ligands: | NAG, GLC, CU and O | ||||||||||||
| Activity: | Laccase, with EC number 1.10.3.2 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
TYPE-2 CU-DEPLETED LACCASE FROM COPRINUS CINEREUS
Overview
Laccase catalyses the oxidation of a variety of organic substrates coupled to the reduction of oxygen to water. It is widely believed to be the simplest representative of the ubiquitous blue multi-copper oxidase family. Laccase is implicated in a wide spectrum of biological activities and, in particular, plays a key role in morphogenesis, development and lignin metabolism in fungi and plants. The structure of laccase from the fungus Coprinus cinereus has been determined by X-ray crystallography at a resolution of 2.2 A. Laccase is a monomer composed of three cupredoxin-like beta-sandwich domains, similar to that found in ascorbate oxidase. In contrast to ascorbate oxidase, however, the mononuclear type-1 Cu site lacks the axial methionine ligand and so exhibits trigonal planar coordination, consistent with its elevated redox potential. Crucially, the structure is trapped in a Cu depleted form in which the putative type-2 Cu atom is completely absent, but in which the remaining type-1 and type-3 Cu sites display full occupancy. Type-2 Cu depletion has unexpected consequences for the coordination of the remaining type-3 Cu atoms.
About this Structure
1A65 is a Single protein structure of sequence from Coprinopsis cinerea. Full crystallographic information is available from OCA.
Reference
Crystal structure of the type-2 Cu depleted laccase from Coprinus cinereus at 2.2 A resolution., Ducros V, Brzozowski AM, Wilson KS, Brown SH, Ostergaard P, Schneider P, Yaver DS, Pedersen AH, Davies GJ, Nat Struct Biol. 1998 Apr;5(4):310-6. PMID:9546223
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