3rpg: Difference between revisions

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[[Image:3rpg.png|left|200px]]
==Bmi1/Ring1b-UbcH5c complex structure==
<StructureSection load='3rpg' size='340' side='right' caption='[[3rpg]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3rpg]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RPG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RPG FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2h0d|2h0d]], [[2ckl|2ckl]], [[2fuh|2fuh]], [[1x23|1x23]]</td></tr>
<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">UBE2D3, UBC5C, UBCH5C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), BMI1, PCGF4, RNF51 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), RNF2, BAP1, DING, HIPI3, RING1B ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span></td></tr>
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rpg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rpg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rpg RCSB], [http://www.ebi.ac.uk/pdbsum/3rpg PDBsum]</span></td></tr>
<table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The Polycomb repressive complex 1 (PRC1) mediates gene silencing, in part by monoubiquitination of histone H2A on lysine 119 (uH2A). Bmi1 and Ring1b are critical components of PRC1 that heterodimerize via their N-terminal RING domains to form an active E3 ubiquitin ligase. We have determined the crystal structure of a complex between the Bmi1/Ring1b RING-RING heterodimer and the E2 enzyme UbcH5c and find that UbcH5c interacts with Ring1b only, in a manner fairly typical of E2-E3 interactions. However, we further show that the Bmi1/Ring1b RING domains bind directly to duplex DNA through a basic surface patch unique to the Bmi1/Ring1b RING-RING dimer. Mutation of residues on this interaction surface leads to a loss of H2A ubiquitination activity. Computational modelling of the interface between Bmi1/Ring1b-UbcH5c and the nucleosome suggests that Bmi1/Ring1b interacts with both nucleosomal DNA and an acidic patch on histone H4 to achieve specific monoubiquitination of H2A. Our results point to a novel mechanism of substrate recognition, and control of product formation, by Bmi1/Ring1b.


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Recognition of UbcH5c and the nucleosome by the Bmi1/Ring1b ubiquitin ligase complex.,Bentley ML, Corn JE, Dong KC, Phung Q, Cheung TK, Cochran AG EMBO J. 2011 Jul 19. doi: 10.1038/emboj.2011.243. PMID:21772249<ref>PMID:21772249</ref>
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{{STRUCTURE_3rpg|  PDB=3rpg  |  SCENE=  }}


===Bmi1/Ring1b-UbcH5c complex structure===
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
</div>


 
==See Also==
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*[[Ubiquitin conjugating enzyme|Ubiquitin conjugating enzyme]]
The line below this paragraph, {{ABSTRACT_PUBMED_21772249}}, adds the Publication Abstract to the page
*[[Ubiquitin protein ligase|Ubiquitin protein ligase]]
(as it appears on PubMed at http://www.pubmed.gov), where 21772249 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_21772249}}
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</StructureSection>
==About this Structure==
[[3rpg]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RPG OCA].
 
==Reference==
<ref group="xtra">PMID:021772249</ref><references group="xtra"/>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Ubiquitin--protein ligase]]
[[Category: Ubiquitin--protein ligase]]

Revision as of 04:51, 5 June 2014

Bmi1/Ring1b-UbcH5c complex structure

3rpg, resolution 2.65Å

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