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{{Seed}}
==PRECURSOR OF THE T152A MUTANT GLYCOSYLASPARAGINASE FROM FLAVOBACTERIUM MENINGOSEPTICUM==
[[Image:9gaa.png|left|200px]]
<StructureSection load='9gaa' size='340' side='right' caption='[[9gaa]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9gaa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Elizabethkingia_meningoseptica Elizabethkingia meningoseptica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9GAA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=9GAA FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.26 3.5.1.26] </span></td></tr>
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=9gaa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9gaa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=9gaa RCSB], [http://www.ebi.ac.uk/pdbsum/9gaa PDBsum]</span></td></tr>
<table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ga/9gaa_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
A variety of proteins, including glycosylasparaginase, have recently been found to activate functions by self-catalyzed peptide bond rearrangements from single-chain precursors. Here we present the 1.9 A crystal structures of glycosylasparaginase precursors that are able to autoproteolyze via an N --&gt; O acyl shift. Several conserved residues are aligned around the scissile peptide bond that is in a highly strained trans peptide bond configuration. The structure illustrates how a nucleophilic side chain may attack the scissile peptide bond at the immediate upstream backbone carbonyl and provides an understanding of the structural basis for peptide bond cleavage via an N --&gt; O or N --&gt; S acyl shift that is used by various groups of intramolecular autoprocessing proteins.


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Structural insights into the mechanism of intramolecular proteolysis.,Xu Q, Buckley D, Guan C, Guo HC Cell. 1999 Sep 3;98(5):651-61. PMID:10490104<ref>PMID:10490104</ref>
The line below this paragraph, containing "STRUCTURE_9gaa", creates the "Structure Box" on the page.
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{{STRUCTURE_9gaa|  PDB=9gaa  |  SCENE=  }}


===PRECURSOR OF THE T152A MUTANT GLYCOSYLASPARAGINASE FROM FLAVOBACTERIUM MENINGOSEPTICUM===
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
 
== References ==
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<references/>
The line below this paragraph, {{ABSTRACT_PUBMED_10490104}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 10490104 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10490104}}
 
==About this Structure==
9GAA is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Elizabethkingia_meningoseptica Elizabethkingia meningoseptica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9GAA OCA].
 
==Reference==
<ref group="xtra">PMID:10490104</ref><references group="xtra"/>
[[Category: Elizabethkingia meningoseptica]]
[[Category: Elizabethkingia meningoseptica]]
[[Category: Guo, H C.]]
[[Category: Guo, H C.]]
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[[Category: Autoproteolysis]]
[[Category: Autoproteolysis]]
[[Category: Glycosylasparaginase]]
[[Category: Glycosylasparaginase]]
[[Category: Hydrolase]]
[[Category: Mutant]]
[[Category: Mutant]]
[[Category: N-terminal nucleophile hydrolase]]
[[Category: N-terminal nucleophile hydrolase]]
[[Category: Precursor]]
[[Category: Precursor]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 18:32:31 2009''
Retrieved from "https://proteopedia.org/9gaa"