1emq: Difference between revisions

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[[~~Image~~:1emq.~~png|left|200px~~]]

==NMR OBSERVATION OF T-TETRADS IN A PARALLEL STRANDED DNA QUADRUPLEX FORMED BY SACCHAROMYCES CEREVISIAE TELOMERE REPEATS==

== Structural highlights ==

<table><tr><td colspan='2'>[[1emq]] is a 4 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EMQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EMQ FirstGlance]. <br>

</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1emq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1emq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1emq RCSB], [http://www.ebi.ac.uk/pdbsum/1emq PDBsum]</span></td></tr>

<table>

== Publication Abstract from PubMed ==

We report here the NMR structure of the DNA sequence d-TGGTGGC containing two repeats of Saccharomyces cerevisiae telomere DNA which is unique in that it has a single thymine in the repeat sequence and the number of Gs can vary from one to three. The structure is a novel quadruplex incor-porating T-tetrads formed by symmetrical pairing of four Ts via O4-H3 H-bonds in a plane. This is in contrast to the previous results on other telomeric sequences which contained more than one T in the repeat sequences and they were seen mostly in the flexible regions of the structures. We observed that the T4-tetrad was nicely accommodated in the center of the G-quadruplex, but it caused a small underwinding of the right handed helix. The T tetrad stacked well on the adjacent G3-tetrad, but poorly on the G5 tetrad. Likewise, T1 also formed a stable T-tetrad at the 5' end of the quadruplex. To our knowledge, this is the first report of T-tetrad formation in DNA structures. These observations are of significance from the points of view of both structural diversity and specific recognitions.

~~{{STRUCTURE_1emq| PDB=1emq | SCENE= }}~~

NMR observation of T-tetrads in a parallel stranded DNA quadruplex formed by Saccharomyces cerevisiae telomere repeats.,Patel PK, Hosur RV Nucleic Acids Res. 1999 Jun 15;27(12):2457-64. PMID:10352174<ref>PMID:10352174</ref>

~~===NMR OBSERVATION OF T-TETRADS IN A PARALLEL STRANDED DNA QUADRUPLEX FORMED BY SACCHAROMYCES CEREVISIAE TELOMERE REPEATS===~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

</div>

~~{{ABSTRACT_PUBMED_10352174}}~~

== References ==

~~==About this Structure==~~

__TOC__

~~[[1emq]] is~~ a ~~4 chain structure. Full experimental information is available from [http://oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=1EMQ OCA].~~

</StructureSection>

==~~Reference~~==

<~~ref group="xtra">PMID:010352174<~~/~~ref~~><~~references group="xtra"~~/>

[[Category: Hosur, R V.]]

[[Category: Patel, P K.]]

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@@ Line 1: / Line 1: @@
-[[Image:1emq.png|left|200px]]
+==NMR OBSERVATION OF T-TETRADS IN A PARALLEL STRANDED DNA QUADRUPLEX FORMED BY SACCHAROMYCES CEREVISIAE TELOMERE REPEATS==
+<StructureSection load='1emq' size='340' side='right' caption='[[1emq]], [[NMR_Ensembles_of_Models | 1 NMR models]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1emq]] is a 4 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EMQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EMQ FirstGlance]. <br>
+</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1emq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1emq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1emq RCSB], [http://www.ebi.ac.uk/pdbsum/1emq PDBsum]</span></td></tr>
+<table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+We report here the NMR structure of the DNA sequence d-TGGTGGC containing two repeats of Saccharomyces cerevisiae telomere DNA which is unique in that it has a single thymine in the repeat sequence and the number of Gs can vary from one to three. The structure is a novel quadruplex incor-porating T-tetrads formed by symmetrical pairing of four Ts via O4-H3 H-bonds in a plane. This is in contrast to the previous results on other telomeric sequences which contained more than one T in the repeat sequences and they were seen mostly in the flexible regions of the structures. We observed that the T4-tetrad was nicely accommodated in the center of the G-quadruplex, but it caused a small underwinding of the right handed helix. The T tetrad stacked well on the adjacent G3-tetrad, but poorly on the G5 tetrad. Likewise, T1 also formed a stable T-tetrad at the 5' end of the quadruplex. To our knowledge, this is the first report of T-tetrad formation in DNA structures. These observations are of significance from the points of view of both structural diversity and specific recognitions.
-{{STRUCTURE_1emq|  PDB=1emq  |  SCENE=  }}
+NMR observation of T-tetrads in a parallel stranded DNA quadruplex formed by Saccharomyces cerevisiae telomere repeats.,Patel PK, Hosur RV Nucleic Acids Res. 1999 Jun 15;27(12):2457-64. PMID:10352174<ref>PMID:10352174</ref>
-===NMR OBSERVATION OF T-TETRADS IN A PARALLEL STRANDED DNA QUADRUPLEX FORMED BY SACCHAROMYCES CEREVISIAE TELOMERE REPEATS===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-{{ABSTRACT_PUBMED_10352174}}
+== References ==
+<references/>
-==About this Structure==
+__TOC__
-[[1emq]] is a 4 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EMQ OCA].
+</StructureSection>
-==Reference==
-<ref group="xtra">PMID:010352174</ref><references group="xtra"/>
 [[Category: Hosur, R V.]]
 [[Category: Patel, P K.]]