1u0i: Difference between revisions
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[[ | ==IAAL-E3/K3 heterodimer== | ||
<StructureSection load='1u0i' size='340' side='right' caption='[[1u0i]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1u0i]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1U0I FirstGlance]. <br> | |||
</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u0i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u0i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1u0i RCSB], [http://www.ebi.ac.uk/pdbsum/1u0i PDBsum]</span></td></tr> | |||
<table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The NMR solution structure of a highly stable coiled-coil IAAL-E3/K3 has been solved. The E3/K3 coiled-coil is a 42-residue de novo designed coiled-coil comprising three heptad repeats per subunit, stabilized by hydrophobic contacts within the core and electrostatic interactions at the interface crossing the hydrophobic core which direct heterodimer formation. This E3/K3 domain has previously been shown to have high alpha-helical content as well as possessing a low dissociation constant (70 nM). The E3/K3 structure is completely alpha-helical and is an archetypical coiled-coil in solution, as determined using a combination of (1)H-NOE and homology based structural restraints. This structure provides a structural framework for visualizing the important interactions for stability and specificity, which are key to protein engineering applications such as affinity purification and de novo design. | |||
NMR solution structure of a highly stable de novo heterodimeric coiled-coil.,Lindhout DA, Litowski JR, Mercier P, Hodges RS, Sykes BD Biopolymers. 2004 Dec 5;75(5):367-75. PMID:15457434<ref>PMID:15457434</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
== | |||
< | |||
[[Category: Hodges, R S.]] | [[Category: Hodges, R S.]] | ||
[[Category: Lindhout, D A.]] | [[Category: Lindhout, D A.]] | ||
