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[[Image:1z2j.png|left|200px]]
==Solution structure of the HIV-1 frameshift inducing element==
<StructureSection load='1z2j' size='340' side='right' caption='[[1z2j]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1z2j]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z2J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Z2J FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1pjy|1pjy]]</td></tr>
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z2j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1z2j RCSB], [http://www.ebi.ac.uk/pdbsum/1z2j PDBsum]</span></td></tr>
<table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Expression of the HIV reverse transcriptase and other essential viral enzymes requires a -1 translational frameshift. The frameshift event is induced by two highly conserved RNA elements within the HIV-1 mRNA: a UUUUUUA heptamer known as the slippery sequence, and a downstream RNA structure. Here, we report structural and thermodynamic evidence that the HIV-1 frameshift site RNA forms a stem-loop and lower helix separated by a three-purine bulge. We have determined the structure of the 45 nucleotide frameshift site RNA using multidimensional heteronuclear nuclear magnetic resonance (NMR) methods. The upper helix is highly thermostable (T(m)&gt;90 degrees C), forming 11 Watson-Crick base-pairs capped by a stable ACAA tetraloop. The eight base-pair lower helix was found to be only moderately stable (T(m)=47 degrees C). A three-purine bulge separates the highly stable upper helix from the lower helix. Base stacking in the bulge forms a wedge, introducing a 60 degrees bend between the helices. Interestingly, this bend is similar to those seen in a number of frameshift inducing pseudoknots for which structures have been solved. The lower helix must denature to allow the ribosome access to the slippery site, but likely functions as a positioning element that enhances frameshift efficiency.


{{STRUCTURE_1z2j|  PDB=1z2j  |  SCENE=  }}
Solution structure and thermodynamic investigation of the HIV-1 frameshift inducing element.,Staple DW, Butcher SE J Mol Biol. 2005 Jun 24;349(5):1011-23. Epub 2005 Apr 1. PMID:15927637<ref>PMID:15927637</ref>


===Solution structure of the HIV-1 frameshift inducing element===
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
{{ABSTRACT_PUBMED_15927637}}
== References ==
 
<references/>
==About this Structure==
__TOC__
[[1z2j]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z2J OCA].
</StructureSection>
 
==Reference==
<ref group="xtra">PMID:015927637</ref><references group="xtra"/>
[[Category: Butcher, S E.]]
[[Category: Butcher, S E.]]
[[Category: Staple, D W.]]
[[Category: Staple, D W.]]
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