1bp7: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1bp7.gif|left|200px]] | [[Image:1bp7.gif|left|200px]] | ||
'''GROUP I MOBILE INTRON ENDONUCLEASE I-CREI COMPLEXED WITH HOMING SITE DNA''' | {{Structure | ||
|PDB= 1bp7 |SIZE=350|CAPTION= <scene name='initialview01'>1bp7</scene>, resolution 3.00Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''GROUP I MOBILE INTRON ENDONUCLEASE I-CREI COMPLEXED WITH HOMING SITE DNA''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
1BP7 is a [ | 1BP7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BP7 OCA]. | ||
==Reference== | ==Reference== | ||
DNA recognition and cleavage by the LAGLIDADG homing endonuclease I-CreI., Jurica MS, Monnat RJ Jr, Stoddard BL, Mol Cell. 1998 Oct;2(4):469-76. PMID:[http:// | DNA recognition and cleavage by the LAGLIDADG homing endonuclease I-CreI., Jurica MS, Monnat RJ Jr, Stoddard BL, Mol Cell. 1998 Oct;2(4):469-76. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9809068 9809068] | ||
[[Category: Chlamydomonas reinhardtii]] | [[Category: Chlamydomonas reinhardtii]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 24: | Line 33: | ||
[[Category: laglidadg motif]] | [[Category: laglidadg motif]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:13:58 2008'' | ||
Revision as of 08:14, 20 March 2008
| |||||||||||||
| 1bp7, resolution 3.00Å | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ligands: | CA | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
GROUP I MOBILE INTRON ENDONUCLEASE I-CREI COMPLEXED WITH HOMING SITE DNA
Overview
The structure of the LAGLIDADG intron-encoded homing endonuclease I-CreI bound to homing site DNA has been determined. The interface is formed by an extended, concave beta sheet from each enzyme monomer that contacts each DNA half-site, resulting in direct side-chain contacts to 18 of the 24 base pairs across the full-length homing site. The structure indicates that I-CreI is optimized to its role in genetic transposition by exhibiting long site-recognition while being able to cleave many closely related target sequences. DNA cleavage is mediated by a compact pair of active sites in the I-CreI homodimer, each of which contains a separate bound divalent cation.
About this Structure
1BP7 is a Single protein structure of sequence from Chlamydomonas reinhardtii. Full crystallographic information is available from OCA.
Reference
DNA recognition and cleavage by the LAGLIDADG homing endonuclease I-CreI., Jurica MS, Monnat RJ Jr, Stoddard BL, Mol Cell. 1998 Oct;2(4):469-76. PMID:9809068
Page seeded by OCA on Thu Mar 20 10:13:58 2008
