1bx5: Difference between revisions
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[[Image:1bx5.gif|left|200px]] | [[Image:1bx5.gif|left|200px]] | ||
'''NMR SOLUTION STRUCTURE OF [D(GCGAAT-3'-3'-ALPHAT-5'-5'-CGC)2]''' | {{Structure | ||
|PDB= 1bx5 |SIZE=350|CAPTION= <scene name='initialview01'>1bx5</scene> | |||
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'''NMR SOLUTION STRUCTURE OF [D(GCGAAT-3'-3'-ALPHAT-5'-5'-CGC)2]''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1BX5 is a [ | 1BX5 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BX5 OCA]. | ||
==Reference== | ==Reference== | ||
NMR solution structures of [d(GCGAAT-3'-3'-alphaT-5'-5'-CGC)2] and its unmodified control., Aramini JM, Mujeeb A, Germann MW, Nucleic Acids Res. 1998 Dec 15;26(24):5644-54. PMID:[http:// | NMR solution structures of [d(GCGAAT-3'-3'-alphaT-5'-5'-CGC)2] and its unmodified control., Aramini JM, Mujeeb A, Germann MW, Nucleic Acids Res. 1998 Dec 15;26(24):5644-54. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9837995 9837995] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Aramini, J M.]] | [[Category: Aramini, J M.]] | ||
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[[Category: Mujeeb, A.]] | [[Category: Mujeeb, A.]] | ||
[[Category: alpha anomeric]] | [[Category: alpha anomeric]] | ||
[[Category: polarity | [[Category: polarity reversal]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:17:03 2008'' | ||
Revision as of 08:17, 20 March 2008
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NMR SOLUTION STRUCTURE OF [D(GCGAAT-3'-3'-ALPHAT-5'-5'-CGC)2]
Overview
We present the high-resolution solution structures of a self-complementary DNA decamer duplex featuring a single alpha-anomeric nucleotide per strand encompassed by a set of 3'-3' and 5'-5' phosphodiester linkages, d(GCGAAT-3'-3'-alphaT-5'-5'-CGC)2, alphaT, and its unmodified control, d(GCGAATTCGC)2, obtained by restrained molecular dynamics. Interproton distance and deoxyribose ring torsion angle restraints were deduced from homonuclear NOESY and DQF-COSY data, respectively. For both the control and alphaT duplexes, excellent global convergence was observed from two different (A- and B-) starting models. The final average structures of the two duplexes are highly homologous, and overall possess the traits characteristic of right-handed B-DNA duplexes. However, localized differences between the two structures stem from the enhanced conformational exchange in the deoxyribose ring of the cytidine following the 5'-5' linkage, the C3'- exo pseudorotation phase angle of the alpha-nucleotide, and unusual backbone torsions in the 3'-3' and 5'-5' phosphodiester linkages. The structural data reported here are relevant to the design of antisense therapeutics comprised of these modifications.
About this Structure
1BX5 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
NMR solution structures of [d(GCGAAT-3'-3'-alphaT-5'-5'-CGC)2] and its unmodified control., Aramini JM, Mujeeb A, Germann MW, Nucleic Acids Res. 1998 Dec 15;26(24):5644-54. PMID:9837995
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