Resolution: Difference between revisions
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'''2.05 Å''' is the '''median''' resolution for [[X-ray crystallography|X-ray crystallographic]] results in the Protein Data Bank (88,701 on May 15, 2014). For comparison, the van der Waals diameter of a carbon atom is 3.4-3.7 Å<ref name="atomsize">See [http://en.wikipedia.org/wiki/Atomic_radii_of_the_elements_(data_page) Atomic radii of the elements (Wikipedia)], and [http://chemwiki.ucdavis.edu/Theoretical_Chemistry/Chemical_Bonding/General_Principles/Covalent_Bond_Distance,_Radius_and_van_der_Waals_Radius values from an Inorganic Chemistry textbook].</ref>, and the length of a covalent carbon-carbon bond is 1.5 Å<ref name="atomsize" />. | '''2.05 Å''' is the '''median''' resolution for [[X-ray crystallography|X-ray crystallographic]] results in the Protein Data Bank (88,701 on May 15, 2014). For comparison, the van der Waals diameter of a carbon atom is 3.4-3.7 Å<ref name="atomsize">See [http://en.wikipedia.org/wiki/Atomic_radii_of_the_elements_(data_page) Atomic radii of the elements (Wikipedia)], and [http://chemwiki.ucdavis.edu/Theoretical_Chemistry/Chemical_Bonding/General_Principles/Covalent_Bond_Distance,_Radius_and_van_der_Waals_Radius values from an Inorganic Chemistry textbook].</ref>, and the length of a covalent carbon-carbon bond is 1.5 Å<ref name="atomsize" />. | ||
If some portions of the macromolecule are less ordered in the crystal than others, these will have a poorer resolution. The "resolution of the crystal" represents the most ordered portions (see [[#Determination of Resolution]]). | |||
After an [[electron density map]] is calculated and refined with a fitted atomic model, an uncertainty of atomic position is calculated for each atom in the model. These single-atom uncertainties are called the B factors or temperature values of the atoms (see [[Temperature]]). | After an [[electron density map]] is calculated and refined with a fitted atomic model, an uncertainty of atomic position is calculated for each atom in the model. These single-atom uncertainties are called the B factors or temperature values of the atoms (see [[Temperature]]). | ||
