1cx5: Difference between revisions
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[[Image:1cx5.gif|left|200px]] | [[Image:1cx5.gif|left|200px]] | ||
'''ANTISENSE DNA/RNA HYBRID CONTAINING MODIFIED BACKBONE''' | {{Structure | ||
|PDB= 1cx5 |SIZE=350|CAPTION= <scene name='initialview01'>1cx5</scene> | |||
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'''ANTISENSE DNA/RNA HYBRID CONTAINING MODIFIED BACKBONE''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1CX5 is a [ | 1CX5 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CX5 OCA]. | ||
==Reference== | ==Reference== | ||
NMR structure of an antisense DNA.RNA hybrid duplex containing a 3'-CH(2)N(CH(3))-O-5' or an MMI backbone linker., Yang X, Han X, Cross C, Bare S, Sanghvi Y, Gao X, Biochemistry. 1999 Sep 28;38(39):12586-96. PMID:[http:// | NMR structure of an antisense DNA.RNA hybrid duplex containing a 3'-CH(2)N(CH(3))-O-5' or an MMI backbone linker., Yang X, Han X, Cross C, Bare S, Sanghvi Y, Gao X, Biochemistry. 1999 Sep 28;38(39):12586-96. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10504227 10504227] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Cross, C.]] | [[Category: Cross, C.]] | ||
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[[Category: nmr]] | [[Category: nmr]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:30:02 2008'' | ||
Revision as of 08:30, 20 March 2008
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ANTISENSE DNA/RNA HYBRID CONTAINING MODIFIED BACKBONE
Overview
The solution structure of an antisense DNA.RNA hybrid duplex, d(CGCGTT-MMI-TTGCGC).r(GCGCAAAACGCG) (designated R4), containing an MMI backbone linker [3'-CH(2)N(CH(3))-O5'], is elucidated. The structural details of the MMI linker, its structural effects on the neighboring residues, and the molecular basis of the MMI effects are examined. The lipophilic N-methyl group of MMI is peripheral to the helix, assuming a conformation that is most stable with regard to the N-O torsion angle. The MMI linker promotes a 3'-endo conformation for the sugar moieties at both 3'- and 5'-adjacent positions and a backbone kink involving distant residues along the 3'-direction. Comparison of R4 with other analogous hybrid duplexes previously studied in this laboratory reveals a new family of low-energy helical conformations that can be accommodated in stable duplexes and a common feature of C3'-modified sugars for adopting a C3'-endo pucker. The results of these studies emphasize the interplay of several factors that govern the formation of stable hybrid duplexes and provide a basis for the understanding of the biological role of the MMI modifications, which are important building blocks for a family of promising chimeric antisense oligonucleotides.
About this Structure
1CX5 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
NMR structure of an antisense DNA.RNA hybrid duplex containing a 3'-CH(2)N(CH(3))-O-5' or an MMI backbone linker., Yang X, Han X, Cross C, Bare S, Sanghvi Y, Gao X, Biochemistry. 1999 Sep 28;38(39):12586-96. PMID:10504227
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