4ou7: Difference between revisions

Line 1:

'''~~Unreleased~~ structure'''

==Crystal structure of DnaT84-153-dT10 ssDNA complex reveals a novel single-stranded DNA binding mode==

== Structural highlights ==

<table><tr><td colspan='2'>[[4ou7]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OU7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OU7 FirstGlance]. <br>

</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ou6|4ou6]]</td></tr>

<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ou7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ou7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ou7 RCSB], [http://www.ebi.ac.uk/pdbsum/4ou7 PDBsum]</span></td></tr>

<table>

== Publication Abstract from PubMed ==

DnaT is a primosomal protein that is required for the stalled replication fork restart in Escherichia coli. As an adapter, DnaT mediates the PriA-PriB-ssDNA ternary complex and the DnaB/C complex. However, the fundamental function of DnaT during PriA-dependent primosome assembly is still a black box. Here, we report the 2.83 A DnaT84-153-dT10 ssDNA complex structure, which reveals a novel three-helix bundle single-stranded DNA binding mode. Based on binding assays and negative-staining electron microscopy results, we found that DnaT can bind to phiX 174 ssDNA to form nucleoprotein filaments for the first time, which indicates that DnaT might function as a scaffold protein during the PriA-dependent primosome assembly. In combination with biochemical analysis, we propose a cooperative mechanism for the binding of DnaT to ssDNA and a possible model for the assembly of PriA-PriB-ssDNA-DnaT complex that sheds light on the function of DnaT during the primosome assembly and stalled replication fork restart. This report presents the first structure of the DnaT C-terminal complex with ssDNA and a novel model that explains the interactions between the three-helix bundle and ssDNA.

~~The entry 4ou7 is ON HOLD until Paper Publication~~

Crystal structure of DnaT84-153-dT10 ssDNA complex reveals a novel single-stranded DNA binding mode.,Liu Z, Chen P, Wang X, Cai G, Niu L, Teng M, Li X Nucleic Acids Res. 2014 Jul 22. pii: gku633. PMID:25053836<ref>PMID:25053836</ref>

~~Authors: Liu~~, Z., Chen, P., Niu, L., Teng, M.~~, Li, X.~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

</div>

~~Description~~: ~~Structure of protein i form 2~~

== References ==

__TOC__

</StructureSection>

[[Category: Chen, P.]]

[[Category: Li, X.]]

[[Category: Liu, Z.]]

[[Category: Niu, L.]]

[[Category: Teng, M.]]

[[Category: Dna binding]]

[[Category: Replication-dna complex]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+==Crystal structure of DnaT84-153-dT10 ssDNA complex reveals a novel single-stranded DNA binding mode==
+<StructureSection load='4ou7' size='340' side='right' caption='[[4ou7]], [[Resolution|resolution]] 2.83&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4ou7]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OU7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OU7 FirstGlance]. <br>
+</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ou6|4ou6]]</td></tr>
+<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ou7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ou7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ou7 RCSB], [http://www.ebi.ac.uk/pdbsum/4ou7 PDBsum]</span></td></tr>
+<table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+DnaT is a primosomal protein that is required for the stalled replication fork restart in Escherichia coli. As an adapter, DnaT mediates the PriA-PriB-ssDNA ternary complex and the DnaB/C complex. However, the fundamental function of DnaT during PriA-dependent primosome assembly is still a black box. Here, we report the 2.83 A DnaT84-153-dT10 ssDNA complex structure, which reveals a novel three-helix bundle single-stranded DNA binding mode. Based on binding assays and negative-staining electron microscopy results, we found that DnaT can bind to phiX 174 ssDNA to form nucleoprotein filaments for the first time, which indicates that DnaT might function as a scaffold protein during the PriA-dependent primosome assembly. In combination with biochemical analysis, we propose a cooperative mechanism for the binding of DnaT to ssDNA and a possible model for the assembly of PriA-PriB-ssDNA-DnaT complex that sheds light on the function of DnaT during the primosome assembly and stalled replication fork restart. This report presents the first structure of the DnaT C-terminal complex with ssDNA and a novel model that explains the interactions between the three-helix bundle and ssDNA.
-The entry 4ou7 is ON HOLD  until Paper Publication
+Crystal structure of DnaT84-153-dT10 ssDNA complex reveals a novel single-stranded DNA binding mode.,Liu Z, Chen P, Wang X, Cai G, Niu L, Teng M, Li X Nucleic Acids Res. 2014 Jul 22. pii: gku633. PMID:25053836<ref>PMID:25053836</ref>
-Authors: Liu, Z., Chen, P., Niu, L., Teng, M., Li, X.
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-Description: Structure of protein i form 2
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Chen, P.]]
+[[Category: Li, X.]]
+[[Category: Liu, Z.]]
+[[Category: Niu, L.]]
+[[Category: Teng, M.]]
+[[Category: Dna binding]]
+[[Category: Replication-dna complex]]