1e4e: Difference between revisions
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'''D-ALANYL-D-LACATE LIGASE''' | {{Structure | ||
|PDB= 1e4e |SIZE=350|CAPTION= <scene name='initialview01'>1e4e</scene>, resolution 2.5Å | |||
|SITE= <scene name='pdbsite=AC1:Phy+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:Phy+Binding+Site+For+Chain+B'>AC2</scene>, <scene name='pdbsite=AC3:Adp+Binding+Site+For+Chain+A'>AC3</scene> and <scene name='pdbsite=AC4:Adp+Binding+Site+For+Chain+B'>AC4</scene> | |||
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=PHY:1(S)-AMINOETHYL-(2-CARBOXYPROPYL)PHOSPHORYL-PHOSPHINIC+ACID'>PHY</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | |||
|ACTIVITY= | |||
|GENE= VANA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1352 Enterococcus faecium]) | |||
}} | |||
'''D-ALANYL-D-LACATE LIGASE''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1E4E is a [ | 1E4E is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Enterococcus_faecium Enterococcus faecium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E4E OCA]. | ||
==Reference== | ==Reference== | ||
The molecular basis of vancomycin resistance in clinically relevant Enterococci: crystal structure of D-alanyl-D-lactate ligase (VanA)., Roper DI, Huyton T, Vagin A, Dodson G, Proc Natl Acad Sci U S A. 2000 Aug 1;97(16):8921-5. PMID:[http:// | The molecular basis of vancomycin resistance in clinically relevant Enterococci: crystal structure of D-alanyl-D-lactate ligase (VanA)., Roper DI, Huyton T, Vagin A, Dodson G, Proc Natl Acad Sci U S A. 2000 Aug 1;97(16):8921-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10908650 10908650] | ||
[[Category: Enterococcus faecium]] | [[Category: Enterococcus faecium]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: plasmid]] | [[Category: plasmid]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:49:28 2008'' | ||
Revision as of 08:49, 20 March 2008
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| 1e4e, resolution 2.5Å | |||||||||||||
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| Sites: | AC1, AC2, AC3 and AC4 | ||||||||||||
| Ligands: | MG, SO4, ADP, PHY and GOL | ||||||||||||
| Gene: | VANA (Enterococcus faecium) | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
D-ALANYL-D-LACATE LIGASE
Overview
d-alanine-d-lactate ligase from Enterococcus faecium BM4147 is directly responsible for the biosynthesis of alternate cell-wall precursors in bacteria, which are resistant to the glycopeptide antibiotic vancomycin. The crystal structure has been determined with data extending to 2.5-A resolution. This structure shows that the active site has unexpected interactions and is distinct from previous models for d-alanyl-d-lactate ligase mechanistic studies. It appears that the preference of the enzyme for lactate as a ligand over d-alanine could be mediated by electrostatic effects and/or a hydrogen-bonding network, which principally involve His-244. The structure of d-alanyl-d-lactate ligase provides a revised interpretation of the molecular events that lead to vancomycin resistance.
About this Structure
1E4E is a Protein complex structure of sequences from Enterococcus faecium. Full crystallographic information is available from OCA.
Reference
The molecular basis of vancomycin resistance in clinically relevant Enterococci: crystal structure of D-alanyl-D-lactate ligase (VanA)., Roper DI, Huyton T, Vagin A, Dodson G, Proc Natl Acad Sci U S A. 2000 Aug 1;97(16):8921-5. PMID:10908650
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