4pla: Difference between revisions
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''' | ==Crystal structure of phosphatidyl inositol 4-kinase II alpha in complex with ATP== | ||
<StructureSection load='4pla' size='340' side='right' caption='[[4pla]], [[Resolution|resolution]] 2.77Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4pla]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PLA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PLA FirstGlance]. <br> | |||
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene><br> | |||
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pla FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pla OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pla RCSB], [http://www.ebi.ac.uk/pdbsum/4pla PDBsum]</span></td></tr> | |||
<table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Phosphoinositides are a class of phospholipids generated by the action of phosphoinositide kinases with key regulatory functions in eukaryotic cells. Here, we present the atomic structure of phosphatidylinositol 4-kinase type IIalpha (PI4K IIalpha), in complex with ATP solved by X-ray crystallography at 2.8 A resolution. The structure revealed a non-typical kinase fold that could be divided into N- and C-lobes with the ATP binding groove located in between. Surprisingly, a second ATP was found in a lateral hydrophobic pocket of the C-lobe. Molecular simulations and mutagenesis analysis revealed the membrane binding mode and the putative function of the hydrophobic pocket. Taken together, our results suggest a mechanism of PI4K IIalpha recruitment, regulation, and function at the membrane. | |||
The | The crystal structure of the phosphatidylinositol 4-kinase IIalpha,Baumlova A, Chalupska D, Rozycki B, Jovic M, Wisniewski E, Klima M, Dubankova A, Kloer DP, Nencka R, Balla T, Boura E EMBO Rep. 2014 Aug 28. pii: e201438841. PMID:25168678<ref>PMID:25168678</ref> | ||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Baumlova, A.]] | |||
[[Category: Boura, E.]] | |||
[[Category: Chalupska, D.]] | |||
[[Category: 4-kinase]] | |||
[[Category: Atp]] | |||
[[Category: Hydrolase]] | |||
[[Category: Lipid]] | |||
[[Category: Phosphatidyl inositol]] | |||
[[Category: Transferase]] | |||
Revision as of 09:52, 10 September 2014
Crystal structure of phosphatidyl inositol 4-kinase II alpha in complex with ATP
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