1ebf: Difference between revisions
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[[Image:1ebf.jpg|left|200px]] | [[Image:1ebf.jpg|left|200px]] | ||
'''HOMOSERINE DEHYDROGENASE FROM S. CEREVISIAE COMPLEX WITH NAD+''' | {{Structure | ||
|PDB= 1ebf |SIZE=350|CAPTION= <scene name='initialview01'>1ebf</scene>, resolution 2.30Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=NA:SODIUM+ION'>NA</scene> and <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Homoserine_dehydrogenase Homoserine dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.3 1.1.1.3] | |||
|GENE= HOM6P ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | |||
}} | |||
'''HOMOSERINE DEHYDROGENASE FROM S. CEREVISIAE COMPLEX WITH NAD+''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1EBF is a [ | 1EBF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EBF OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structures of homoserine dehydrogenase suggest a novel catalytic mechanism for oxidoreductases., DeLaBarre B, Thompson PR, Wright GD, Berghuis AM, Nat Struct Biol. 2000 Mar;7(3):238-44. PMID:[http:// | Crystal structures of homoserine dehydrogenase suggest a novel catalytic mechanism for oxidoreductases., DeLaBarre B, Thompson PR, Wright GD, Berghuis AM, Nat Struct Biol. 2000 Mar;7(3):238-44. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10700284 10700284] | ||
[[Category: Homoserine dehydrogenase]] | [[Category: Homoserine dehydrogenase]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
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[[Category: nad]] | [[Category: nad]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:53:10 2008'' | ||
Revision as of 08:53, 20 March 2008
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| 1ebf, resolution 2.30Å | |||||||||||||
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| Ligands: | NA and NAD | ||||||||||||
| Gene: | HOM6P (Saccharomyces cerevisiae) | ||||||||||||
| Activity: | Homoserine dehydrogenase, with EC number 1.1.1.3 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
HOMOSERINE DEHYDROGENASE FROM S. CEREVISIAE COMPLEX WITH NAD+
Overview
The structure of the antifungal drug target homoserine dehydrogenase (HSD) was determined from Saccharomyces cerevisiae in apo and holo forms, and as a ternary complex with bound products, by X-ray diffraction. The three forms show that the enzyme is a dimer, with each monomer composed of three regions, the nucleotide-binding region, the dimerization region and the catalytic region. The dimerization and catalytic regions have novel folds, whereas the fold of the nucleotide-binding region is a variation on the Rossmann fold. The novel folds impose a novel composition and arrangement of active site residues when compared to all other currently known oxidoreductases. This observation, in conjunction with site-directed mutagenesis of active site residues and steady-state kinetic measurements, suggest that HSD exhibits a new variation on dehydrogenase chemistry.
About this Structure
1EBF is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Crystal structures of homoserine dehydrogenase suggest a novel catalytic mechanism for oxidoreductases., DeLaBarre B, Thompson PR, Wright GD, Berghuis AM, Nat Struct Biol. 2000 Mar;7(3):238-44. PMID:10700284
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