4mi7: Difference between revisions

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~~{{STRUCTURE_4mi7~~| ~~PDB~~=4mi7 | ~~SCENE~~= }}

==Crystal Structure of Salmonella Effector Protein GtgE==

===~~Crystal Structure~~ of Salmonella ~~Effector Protein~~ GtgE~~===~~

== Structural highlights ==

<table><tr><td colspan='2'>[[4mi7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Salts Salts]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MI7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MI7 FirstGlance]. <br>

</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>

<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gtgE, SL1344_0995 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=216597 SALTS])</td></tr>

<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mi7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mi7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mi7 RCSB], [http://www.ebi.ac.uk/pdbsum/4mi7 PDBsum]</span></td></tr>

<table>

== Publication Abstract from PubMed ==

GtgE is an effector protein from Salmonella Typhimurium that modulates trafficking of the Salmonella-containing vacuole. It exerts its function by cleaving the Rab-family GTPases Rab29, Rab32 and Rab38, thereby preventing the delivery of antimicrobial factors to the bacteria-containing vacuole. Here, the crystal structure of GtgE at 1.65 A resolution is presented, and structure-based mutagenesis and in vivo infection assays are used to identify its catalytic triad. A panel of cysteine protease inhibitors were examined and it was determined that N-ethylmaleimide, antipain and chymostatin inhibit GtgE activity in vitro. These findings provide the basis for the development of novel therapeutic strategies to combat Salmonella infections.

~~==About this Structure==~~

Structural and enzymatic characterization of a host-specificity determinant from Salmonella.,Kohler AC, Spano S, Galan JE, Stebbins CE Acta Crystallogr D Biol Crystallogr. 2014 Feb;70(Pt 2):384-91. doi:, 10.1107/S1399004713028393. Epub 2014 Jan 29. PMID:24531472<ref>PMID:24531472</ref>

~~[[4mi7]] is~~ a ~~1 chain structure~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin~~/~~ocashort?id=4MI7 OCA~~].

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

</div>

== References ==

__TOC__

</StructureSection>

[[Category: Salts]]

[[Category: Kohler, A C.]]

[[Category: Stebbins, C E.]]

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-{{STRUCTURE_4mi7|  PDB=4mi7  |  SCENE=  }}
+==Crystal Structure of Salmonella Effector Protein GtgE==
-===Crystal Structure of Salmonella Effector Protein GtgE===
+<StructureSection load='4mi7' size='340' side='right' caption='[[4mi7]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4mi7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Salts Salts]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MI7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MI7 FirstGlance]. <br>
+</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
+<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gtgE, SL1344_0995 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=216597 SALTS])</td></tr>
+<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mi7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mi7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mi7 RCSB], [http://www.ebi.ac.uk/pdbsum/4mi7 PDBsum]</span></td></tr>
+<table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+GtgE is an effector protein from Salmonella Typhimurium that modulates trafficking of the Salmonella-containing vacuole. It exerts its function by cleaving the Rab-family GTPases Rab29, Rab32 and Rab38, thereby preventing the delivery of antimicrobial factors to the bacteria-containing vacuole. Here, the crystal structure of GtgE at 1.65 A resolution is presented, and structure-based mutagenesis and in vivo infection assays are used to identify its catalytic triad. A panel of cysteine protease inhibitors were examined and it was determined that N-ethylmaleimide, antipain and chymostatin inhibit GtgE activity in vitro. These findings provide the basis for the development of novel therapeutic strategies to combat Salmonella infections.
-==About this Structure==
+Structural and enzymatic characterization of a host-specificity determinant from Salmonella.,Kohler AC, Spano S, Galan JE, Stebbins CE Acta Crystallogr D Biol Crystallogr. 2014 Feb;70(Pt 2):384-91. doi:, 10.1107/S1399004713028393. Epub 2014 Jan 29. PMID:24531472<ref>PMID:24531472</ref>
-[[4mi7]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MI7 OCA].
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Salts]]
 [[Category: Kohler, A C.]]
 [[Category: Stebbins, C E.]]