1ej1: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1ej1.jpg|left|200px]] | [[Image:1ej1.jpg|left|200px]] | ||
'''COCRYSTAL STRUCTURE OF THE MESSENGER RNA 5' CAP-BINDING PROTEIN (EIF4E) BOUND TO 7-METHYL-GDP''' | {{Structure | ||
|PDB= 1ej1 |SIZE=350|CAPTION= <scene name='initialview01'>1ej1</scene>, resolution 2.2Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=M7G:7N-METHYL-8-HYDROGUANOSINE-5'-DIPHOSPHATE'>M7G</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''COCRYSTAL STRUCTURE OF THE MESSENGER RNA 5' CAP-BINDING PROTEIN (EIF4E) BOUND TO 7-METHYL-GDP''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
1EJ1 is a [ | 1EJ1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EJ1 OCA]. | ||
==Reference== | ==Reference== | ||
Cocrystal structure of the messenger RNA 5' cap-binding protein (eIF4E) bound to 7-methyl-GDP., Marcotrigiano J, Gingras AC, Sonenberg N, Burley SK, Cell. 1997 Jun 13;89(6):951-61. PMID:[http:// | Cocrystal structure of the messenger RNA 5' cap-binding protein (eIF4E) bound to 7-methyl-GDP., Marcotrigiano J, Gingras AC, Sonenberg N, Burley SK, Cell. 1997 Jun 13;89(6):951-61. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9200613 9200613] | ||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 20: | Line 29: | ||
[[Category: protein 5' mrna cap complex]] | [[Category: protein 5' mrna cap complex]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:56:17 2008'' | ||
Revision as of 08:56, 20 March 2008
| |||||||||||||
| 1ej1, resolution 2.2Å | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ligands: | M7G | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
COCRYSTAL STRUCTURE OF THE MESSENGER RNA 5' CAP-BINDING PROTEIN (EIF4E) BOUND TO 7-METHYL-GDP
Overview
The X-ray structure of the eukaryotic translation initiation factor 4E (eIF4E), bound to 7-methyl-GDP, has been determined at 2.2 A resolution. eIF4E recognizes 5' 7-methyl-G(5')ppp(5')N mRNA caps during the rate-limiting initiation step of translation. The protein resembles a cupped hand and consists of a curved, 8-stranded antiparallel beta sheet, backed by three long alpha helices. 7-methyl-GDP binds in a narrow cap-binding slot on the molecule's concave surface, where 7-methyl-guanine recognition is mediated by base sandwiching between two conserved tryptophans, plus formation of three hydrogen bonds and a van der Waals contact between its N7-methyl group and a third conserved tryptophan. The convex dorsal surface of the molecule displays a phylogenetically conserved hydrophobic/acidic portion, which may interact with other translation initiation factors and regulatory proteins.
About this Structure
1EJ1 is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Cocrystal structure of the messenger RNA 5' cap-binding protein (eIF4E) bound to 7-methyl-GDP., Marcotrigiano J, Gingras AC, Sonenberg N, Burley SK, Cell. 1997 Jun 13;89(6):951-61. PMID:9200613
Page seeded by OCA on Thu Mar 20 10:56:17 2008
