1erj: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1erj.jpg|left|200px]] | [[Image:1erj.jpg|left|200px]] | ||
'''CRYSTAL STRUCTURE OF THE C-TERMINAL WD40 DOMAIN OF TUP1''' | {{Structure | ||
|PDB= 1erj |SIZE=350|CAPTION= <scene name='initialview01'>1erj</scene>, resolution 2.3Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''CRYSTAL STRUCTURE OF THE C-TERMINAL WD40 DOMAIN OF TUP1''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
1ERJ is a [ | 1ERJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ERJ OCA]. | ||
==Reference== | ==Reference== | ||
Structure of the C-terminal domain of Tup1, a corepressor of transcription in yeast., Sprague ER, Redd MJ, Johnson AD, Wolberger C, EMBO J. 2000 Jun 15;19(12):3016-27. PMID:[http:// | Structure of the C-terminal domain of Tup1, a corepressor of transcription in yeast., Sprague ER, Redd MJ, Johnson AD, Wolberger C, EMBO J. 2000 Jun 15;19(12):3016-27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10856245 10856245] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 19: | Line 28: | ||
[[Category: beta-propeller]] | [[Category: beta-propeller]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:59:22 2008'' | ||
Revision as of 08:59, 20 March 2008
| |||||||||||||
| 1erj, resolution 2.3Å | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
CRYSTAL STRUCTURE OF THE C-TERMINAL WD40 DOMAIN OF TUP1
Overview
The Tup1-Ssn6 corepressor complex regulates the expression of several sets of genes, including genes that specify mating type in the yeast Saccharomyces cerevisiae. Repression of mating-type genes occurs when Tup1-Ssn6 is brought to the DNA by the Matalpha2 DNA-binding protein and assembled upstream of a- and haploid-specific genes. We have determined the 2.3 A X-ray crystal structure of the C-terminal domain of Tup1 (accesion No. 1ERJ), a 43 kDa fragment that contains seven copies of the WD40 sequence motif and binds to the Matalpha2 protein. Moreover, this portion of the protein can partially substitute for full-length Tup1 in bringing about transcriptional repression. The structure reveals a seven-bladed beta propeller with an N-terminal subdomain that is anchored to the side of the propeller and extends the beta sheet of one of the blades. Point mutations in Tup1 that specifically affect the Tup1-Matalpha2 interaction cluster on one surface of the propeller. We identified regions of Tup1 that are conserved among the fungal Tup1 homologs and may be important in protein-protein interactions with additional components of the Tup1-mediated repression pathways.
About this Structure
1ERJ is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Structure of the C-terminal domain of Tup1, a corepressor of transcription in yeast., Sprague ER, Redd MJ, Johnson AD, Wolberger C, EMBO J. 2000 Jun 15;19(12):3016-27. PMID:10856245
Page seeded by OCA on Thu Mar 20 10:59:22 2008
