1ma8: Difference between revisions

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[[~~Image~~:1ma8.~~png|left|200px~~]]

==A-DNA decamer GCGTA(UMS)ACGC with incorporated 2'-methylseleno-uridine==

== Structural highlights ==

<table><tr><td colspan='2'>[[1ma8]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MA8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MA8 FirstGlance]. <br>

</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UMS:2-METHYLSELENYL-2-DEOXYURIDINE-5-PHOSPHATE'>UMS</scene></td></tr>

<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ma8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ma8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ma8 RCSB], [http://www.ebi.ac.uk/pdbsum/1ma8 PDBsum]</span></td></tr>

<table>

== Publication Abstract from PubMed ==

Selenium was incorporated into an oligodeoxynucleotide in the form of 2'-methylseleno-uridine (U(Se)). The X-ray crystal structure of the duplex left open bracket d(GCGTA)U(Se)d(ACGC) right open bracket (2) was determined by the multiwavelength anomalous dispersion (MAD) technique and refined to a resolution of 1.3 A, demonstrating that selenium can selectively substitute oxygen in DNA and that the resulting compounds are chemically stable. Since derivatization at the 2'-alpha-position with selenium does not affect the preference of the sugar for the C3'-endo conformation, this strategy is suitable for incorporating selenium into RNA. The availability of selenium-containing nucleic acids for crystallographic phasing offers an attractive alternative to the commonly used halogenated pyrimidines.

~~{{STRUCTURE_1ma8| PDB=1ma8 | SCENE= }}~~

Covalent incorporation of selenium into oligonucleotides for X-ray crystal structure determination via MAD: proof of principle. Multiwavelength anomalous dispersion.,Teplova M, Wilds CJ, Wawrzak Z, Tereshko V, Du Q, Carrasco N, Huang Z, Egli M Biochimie. 2002 Sep;84(9):849-58. PMID:12458077<ref>PMID:12458077</ref>

~~===A-DNA decamer GCGTA(UMS)ACGC with incorporated 2'-methylseleno-uridine===~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

</div>

~~{{ABSTRACT_PUBMED_12458077}}~~

== References ==

==~~About this Structure~~==

__TOC__

~~[[1ma8]] is a 2 chain structure. Full crystallographic information is available from [http:~~//~~oca.weizmann.ac.il/oca-bin/ocashort?id=1MA8 OCA].~~

</StructureSection>

[[Category: Carrasco, N.]]

[[Category: Du, Q.]]

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@@ Line 1: / Line 1: @@
-[[Image:1ma8.png|left|200px]]
+==A-DNA decamer GCGTA(UMS)ACGC with incorporated 2'-methylseleno-uridine==
+<StructureSection load='1ma8' size='340' side='right' caption='[[1ma8]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1ma8]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MA8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MA8 FirstGlance]. <br>
+</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UMS:2-METHYLSELENYL-2-DEOXYURIDINE-5-PHOSPHATE'>UMS</scene></td></tr>
+<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ma8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ma8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ma8 RCSB], [http://www.ebi.ac.uk/pdbsum/1ma8 PDBsum]</span></td></tr>
+<table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Selenium was incorporated into an oligodeoxynucleotide in the form of 2'-methylseleno-uridine (U(Se)). The X-ray crystal structure of the duplex left open bracket d(GCGTA)U(Se)d(ACGC) right open bracket (2) was determined by the multiwavelength anomalous dispersion (MAD) technique and refined to a resolution of 1.3 A, demonstrating that selenium can selectively substitute oxygen in DNA and that the resulting compounds are chemically stable. Since derivatization at the 2'-alpha-position with selenium does not affect the preference of the sugar for the C3'-endo conformation, this strategy is suitable for incorporating selenium into RNA. The availability of selenium-containing nucleic acids for crystallographic phasing offers an attractive alternative to the commonly used halogenated pyrimidines.
-{{STRUCTURE_1ma8|  PDB=1ma8  |  SCENE=  }}
+Covalent incorporation of selenium into oligonucleotides for X-ray crystal structure determination via MAD: proof of principle. Multiwavelength anomalous dispersion.,Teplova M, Wilds CJ, Wawrzak Z, Tereshko V, Du Q, Carrasco N, Huang Z, Egli M Biochimie. 2002 Sep;84(9):849-58. PMID:12458077<ref>PMID:12458077</ref>
-===A-DNA decamer GCGTA(UMS)ACGC with incorporated 2'-methylseleno-uridine===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-{{ABSTRACT_PUBMED_12458077}}
+== References ==
+<references/>
-==About this Structure==
+__TOC__
-[[1ma8]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MA8 OCA].
+</StructureSection>
 [[Category: Carrasco, N.]]
 [[Category: Du, Q.]]