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'''CRYSTAL STRUCTURE OF SMAC/DIABLO''' | {{Structure | ||
|PDB= 1few |SIZE=350|CAPTION= <scene name='initialview01'>1few</scene>, resolution 2.2Å | |||
|SITE= | |||
|LIGAND= | |||
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'''CRYSTAL STRUCTURE OF SMAC/DIABLO''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1FEW is a [ | 1FEW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FEW OCA]. | ||
==Reference== | ==Reference== | ||
Structural and biochemical basis of apoptotic activation by Smac/DIABLO., Chai J, Du C, Wu JW, Kyin S, Wang X, Shi Y, Nature. 2000 Aug 24;406(6798):855-62. PMID:[http:// | Structural and biochemical basis of apoptotic activation by Smac/DIABLO., Chai J, Du C, Wu JW, Kyin S, Wang X, Shi Y, Nature. 2000 Aug 24;406(6798):855-62. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10972280 10972280] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: smac]] | [[Category: smac]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:08:16 2008'' | ||
Revision as of 09:08, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
CRYSTAL STRUCTURE OF SMAC/DIABLO
Overview
Apoptosis (programmed cell death), an essential process in the development and homeostasis of metazoans, is carried out by caspases. The mitochondrial protein Smac/DIABLO performs a critical function in apoptosis by eliminating the inhibitory effect of IAPs (inhibitor of apoptosis proteins) on caspases. Here we show that Smac/DIABLO promotes not only the proteolytic activation of procaspase-3 but also the enzymatic activity of mature caspase-3, both of which depend upon its ability to interact physically with IAPs. The crystal structure of Smac/DIABLO at 2.2 A resolution reveals that it homodimerizes through an extensive hydrophobic interface. Missense mutations inactivating this dimeric interface significantly compromise the function of Smac/DIABLO. As in the Drosophila proteins Reaper, Grim and Hid, the amino-terminal amino acids of Smac/DIABLO are indispensable for its function, and a seven-residue peptide derived from the amino terminus promotes procaspase-3 activation in vitro. These results establish an evolutionarily conserved structural and biochemical basis for the activation of apoptosis by Smac/DIABLO.
About this Structure
1FEW is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural and biochemical basis of apoptotic activation by Smac/DIABLO., Chai J, Du C, Wu JW, Kyin S, Wang X, Shi Y, Nature. 2000 Aug 24;406(6798):855-62. PMID:10972280
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