1p9f: Difference between revisions

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[[~~Image~~:1p9f.~~png|left|200px~~]]

==NMR Structure of Neurokinin B from DYANA==

== Structural highlights ==

<table><tr><td colspan='2'>[[1p9f]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P9F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1P9F FirstGlance]. <br>

</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1p9f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p9f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1p9f RCSB], [http://www.ebi.ac.uk/pdbsum/1p9f PDBsum]</span></td></tr>

<table>

== Publication Abstract from PubMed ==

Neurokinin B (NKB), a decapeptide of mammalian origin exhibits a variety of biological activities such as regulatory functions in reproduction, pre-eclampsia and neuroprotection in Alzheimer's disease. In order to gain insight into structure-function relationship, three-dimensional structure of NKB has been investigated using CD spectropolarimetry and two-dimensional proton nuclear magnetic resonance (2D 1H-NMR) spectroscopy in aqueous and membrane mimetic solvents. Unambiguous NMR assignments of resonances have been made with the aid of correlation spectroscopy (DQF-COSY and TOCSY) experiments and Nuclear Overhauser Effect Spectroscopy (NOESY) experiments. Distance constraints obtained from the NMR data have been used to generate a family of structures, which have been refined using restrained energy minimization and dynamics. Our data show that a helical structure is induced in NKB, in presence of perdeuterated dodecyl phosphocholine (DPC) micelles, a membrane model system. Further, the conformation adopted by NKB in presence of DPC micelles represents a structural motif typical of neurokinin-3 selective agonists.

~~{{STRUCTURE_1p9f| PDB=1p9f | SCENE= }}~~

Three dimensional structure of mammalian tachykinin peptide neurokinin B bound to lipid micelles.,Mantha AK, Chandrashekar IR, Baquer NZ, Cowsik SM J Biomol Struct Dyn. 2004 Oct;22(2):137-48. PMID:15317475<ref>PMID:15317475</ref>

~~===NMR Structure~~ of ~~Neurokinin B from DYANA===~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

</div>

~~{{ABSTRACT_PUBMED_15317475}}~~

== References ==

==~~About this Structure~~==

__TOC__

~~[[1p9f]] is a 1 chain structure. Full experimental information is available from [http:~~//~~oca.weizmann.ac.il/oca-bin/ocashort?id=1P9F OCA].~~

</StructureSection>

[[Category: Baquer, N Z.]]

[[Category: Chandrashekar, I R.]]

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-[[Image:1p9f.png|left|200px]]
+==NMR Structure of Neurokinin B from DYANA==
+<StructureSection load='1p9f' size='340' side='right' caption='[[1p9f]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1p9f]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P9F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1P9F FirstGlance]. <br>
+</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1p9f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p9f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1p9f RCSB], [http://www.ebi.ac.uk/pdbsum/1p9f PDBsum]</span></td></tr>
+<table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Neurokinin B (NKB), a decapeptide of mammalian origin exhibits a variety of biological activities such as regulatory functions in reproduction, pre-eclampsia and neuroprotection in Alzheimer's disease. In order to gain insight into structure-function relationship, three-dimensional structure of NKB has been investigated using CD spectropolarimetry and two-dimensional proton nuclear magnetic resonance (2D 1H-NMR) spectroscopy in aqueous and membrane mimetic solvents. Unambiguous NMR assignments of resonances have been made with the aid of correlation spectroscopy (DQF-COSY and TOCSY) experiments and Nuclear Overhauser Effect Spectroscopy (NOESY) experiments. Distance constraints obtained from the NMR data have been used to generate a family of structures, which have been refined using restrained energy minimization and dynamics. Our data show that a helical structure is induced in NKB, in presence of perdeuterated dodecyl phosphocholine (DPC) micelles, a membrane model system. Further, the conformation adopted by NKB in presence of DPC micelles represents a structural motif typical of neurokinin-3 selective agonists.
-{{STRUCTURE_1p9f|  PDB=1p9f  |  SCENE=  }}
+Three dimensional structure of mammalian tachykinin peptide neurokinin B bound to lipid micelles.,Mantha AK, Chandrashekar IR, Baquer NZ, Cowsik SM J Biomol Struct Dyn. 2004 Oct;22(2):137-48. PMID:15317475<ref>PMID:15317475</ref>
-===NMR Structure of Neurokinin B from DYANA===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-{{ABSTRACT_PUBMED_15317475}}
+== References ==
+<references/>
-==About this Structure==
+__TOC__
-[[1p9f]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P9F OCA].
+</StructureSection>
 [[Category: Baquer, N Z.]]
 [[Category: Chandrashekar, I R.]]