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[[Image:2jkx.png|left|200px]]
==GALACTOSE OXIDASE. MATGO. COPPER FREE, EXPRESSED IN PICHIA PASTORIS.==
<StructureSection load='2jkx' size='340' side='right' caption='[[2jkx]], [[Resolution|resolution]] 1.84&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2jkx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gibberella_zeae Gibberella zeae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JKX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JKX FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene><br>
<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1t2x|1t2x]], [[1goh|1goh]], [[2vz3|2vz3]], [[1gof|1gof]], [[2vz1|2vz1]], [[1k3i|1k3i]], [[1gog|1gog]]</td></tr>
<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Galactose_oxidase Galactose oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.3.9 1.1.3.9] </span></td></tr>
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jkx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jkx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2jkx RCSB], [http://www.ebi.ac.uk/pdbsum/2jkx PDBsum]</span></td></tr>
<table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jk/2jkx_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Galactose oxidase (GO; EC 1.1.3.9) catalyses the oxidation of a wide range of primary alcohols including mono-, oligo- and polysaccharides. High-resolution structures have been determined for GO, but no structural information is available for the enzyme with bound substrate or inhibitor. Previously, computer-aided docking experiments have been used to develop a plausible model for interactions between GO and the D-galactose substrate. Residues implicated in such interactions include Arg330, Gln406, Phe464, Phe194 and Trp290. In the present study we describe an improved expression system for recombinant GO in the methylotrophic yeast Pichia pastoris. We use this system to express variant proteins mutated at Arg330 and Phe464 to explore the substrate binding model. We also demonstrate that the Arg330 variants display greater fructose oxidase activity than does wild-type GO.


{{STRUCTURE_2jkx|  PDB=2jkx  |  SCENE=  }}
Enhanced fructose oxidase activity in a galactose oxidase variant.,Deacon SE, Mahmoud K, Spooner RK, Firbank SJ, Knowles PF, Phillips SE, McPherson MJ Chembiochem. 2004 Jul 5;5(7):972-9. PMID:15239055<ref>PMID:15239055</ref>


===GALACTOSE OXIDASE. MATGO. COPPER FREE, EXPRESSED IN PICHIA PASTORIS.===
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
{{ABSTRACT_PUBMED_15239055}}
 
==About this Structure==
[[2jkx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gibberella_zeae Gibberella zeae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JKX OCA].


==See Also==
==See Also==
*[[Galactose oxidase|Galactose oxidase]]
*[[Galactose oxidase|Galactose oxidase]]
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:015239055</ref><references group="xtra"/>
__TOC__
</StructureSection>
[[Category: Galactose oxidase]]
[[Category: Galactose oxidase]]
[[Category: Gibberella zeae]]
[[Category: Gibberella zeae]]
Retrieved from "https://proteopedia.org/2jkx"