3cvz: Difference between revisions

Line 1:

[[Image:~~3cvz~~.~~png~~|~~left~~|~~200px~~]]

==Structural insights into the molecular organization of the S-layer from Clostridium difficile==

== Structural highlights ==

<table><tr><td colspan='2'>[[3cvz]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/[clostridium]_difficile [clostridium] difficile]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CVZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CVZ FirstGlance]. <br>

</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLY:GLYCINE'>GLY</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>

<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">slpA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1496 [Clostridium] difficile])</td></tr>

<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cvz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cvz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3cvz RCSB], [http://www.ebi.ac.uk/pdbsum/3cvz PDBsum]</span></td></tr>

<table>

== Evolutionary Conservation ==

[[Image:Consurf_key_small.gif|200px|right]]

Check<jmol>

<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cv/3cvz_consurf.spt"</scriptWhenChecked>

<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>

<text>to colour the structure by Evolutionary Conservation</text>

</jmolCheckbox>

</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].

== Publication Abstract from PubMed ==

Clostridium difficile expresses a surface layer (S-layer) which coats the surface of the bacterium and acts as an adhesin facilitating interaction of the bacterium with host enteric cells. The S-layer contains a high-molecular-weight S-layer protein (HMW SLP) and its low-molecular-weight partner protein (LMW SLP). We show that these proteins form a tightly associated non-covalent complex, the H/L complex, and we identify the regions of both proteins responsible for complex formation. The 2.4 A X-ray crystal structure of a truncated derivative of the LMW SLP reveals two domains. Domain 1 has a two-layer sandwich architecture while domain 2, predicted to orientate towards the external environment, contains a novel fold. Small-angle X-ray scattering analysis of the H/L complex shows an elongated molecule, with the two SLPs arranged 'end-to-end' interacting with each other through a small contact area. Alignment of LMW SLPs, which exhibit high sequence diversity, reveals a core of conserved residues that could reflect functional conservation, while allowing for immune evasion through sequence variation. These structures are the first described for the S-layer of a bacterial pathogen, and provide insights into the assembly and biogenesis of the S-layer.

~~{{STRUCTURE_3cvz| PDB=3cvz | SCENE= }}~~

Structural insights into the molecular organization of the S-layer from Clostridium difficile.,Fagan RP, Albesa-Jove D, Qazi O, Svergun DI, Brown KA, Fairweather NF Mol Microbiol. 2009 Mar;71(5):1308-22. Epub 2009 Jan 29. PMID:19183279<ref>PMID:19183279</ref>

~~===Structural insights into the molecular organization~~ of the S~~-layer from Clostridium difficile===~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

</div>

~~{{ABSTRACT_PUBMED_19183279}}~~

== References ==

==~~About this Structure~~==

__TOC__

~~[[3cvz]] is a 4 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/[clostridium]_difficile [clostridium] difficile]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CVZ OCA].~~

</StructureSection>

[[Category: Albesa-Jove, D.]]

[[Category: Fagan, R.]]

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@@ Line 1: / Line 1: @@
-[[Image:3cvz.png|left|200px]]
+==Structural insights into the molecular organization of the S-layer from Clostridium difficile==
+<StructureSection load='3cvz' size='340' side='right' caption='[[3cvz]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3cvz]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/[clostridium]_difficile [clostridium] difficile]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CVZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CVZ FirstGlance]. <br>
+</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLY:GLYCINE'>GLY</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>
+<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">slpA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1496 [Clostridium] difficile])</td></tr>
+<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cvz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cvz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3cvz RCSB], [http://www.ebi.ac.uk/pdbsum/3cvz PDBsum]</span></td></tr>
+<table>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cv/3cvz_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Clostridium difficile expresses a surface layer (S-layer) which coats the surface of the bacterium and acts as an adhesin facilitating interaction of the bacterium with host enteric cells. The S-layer contains a high-molecular-weight S-layer protein (HMW SLP) and its low-molecular-weight partner protein (LMW SLP). We show that these proteins form a tightly associated non-covalent complex, the H/L complex, and we identify the regions of both proteins responsible for complex formation. The 2.4 A X-ray crystal structure of a truncated derivative of the LMW SLP reveals two domains. Domain 1 has a two-layer sandwich architecture while domain 2, predicted to orientate towards the external environment, contains a novel fold. Small-angle X-ray scattering analysis of the H/L complex shows an elongated molecule, with the two SLPs arranged 'end-to-end' interacting with each other through a small contact area. Alignment of LMW SLPs, which exhibit high sequence diversity, reveals a core of conserved residues that could reflect functional conservation, while allowing for immune evasion through sequence variation. These structures are the first described for the S-layer of a bacterial pathogen, and provide insights into the assembly and biogenesis of the S-layer.
-{{STRUCTURE_3cvz|  PDB=3cvz  |  SCENE=  }}
+Structural insights into the molecular organization of the S-layer from Clostridium difficile.,Fagan RP, Albesa-Jove D, Qazi O, Svergun DI, Brown KA, Fairweather NF Mol Microbiol. 2009 Mar;71(5):1308-22. Epub 2009 Jan 29. PMID:19183279<ref>PMID:19183279</ref>
-===Structural insights into the molecular organization of the S-layer from Clostridium difficile===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-{{ABSTRACT_PUBMED_19183279}}
+== References ==
+<references/>
-==About this Structure==
+__TOC__
-[[3cvz]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/[clostridium]_difficile [clostridium] difficile]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CVZ OCA].
+</StructureSection>
 [[Category: Albesa-Jove, D.]]
 [[Category: Fagan, R.]]