3ep2: Difference between revisions

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[[Image:3ep2.png|left|200px]]
==Model of Phe-tRNA(Phe) in the ribosomal pre-accommodated state revealed by cryo-EM==
<StructureSection load='3ep2' size='340' side='right' caption='[[3ep2]], [[Resolution|resolution]] 9.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3ep2]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EP2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EP2 FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2avy|2avy]], [[2aw4|2aw4]], [[1qza|1qza]], [[1ob2|1ob2]], [[3eq3|3eq3]], [[3eq4|3eq4]]</td></tr>
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ep2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ep2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ep2 RCSB], [http://www.ebi.ac.uk/pdbsum/3ep2 PDBsum]</span></td></tr>
<table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ep/3ep2_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The accuracy of ribosomal translation is achieved by an initial selection and a proofreading step, mediated by EF-Tu, which forms a ternary complex with aminoacyl(aa)-tRNA. To study the binding modes of different aa-tRNAs, we compared cryo-EM maps of the kirromycin-stalled ribosome bound with ternary complexes containing Phe-tRNA(Phe), Trp-tRNA(Trp), or Leu-tRNA(LeuI). The three maps suggest a common binding manner of cognate aa-tRNAs in their specific binding with both the ribosome and EF-Tu. All three aa-tRNAs have the same 'loaded spring' conformation with a kink and twist between the D-stem and anticodon stem. The three complexes are similarly integrated in an interaction network, extending from the anticodon loop through h44 and protein S12 to the EF-Tu-binding CCA end of aa-tRNA, proposed to signal cognate codon-anticodon interaction to the GTPase centre and tune the accuracy of aa-tRNA selection.


{{STRUCTURE_3ep2|  PDB=3ep2  |  SCENE=  }}
Recognition of aminoacyl-tRNA: a common molecular mechanism revealed by cryo-EM.,Li W, Agirrezabala X, Lei J, Bouakaz L, Brunelle JL, Ortiz-Meoz RF, Green R, Sanyal S, Ehrenberg M, Frank J EMBO J. 2008 Dec 17;27(24):3322-31. Epub 2008 Nov 20. PMID:19020518<ref>PMID:19020518</ref>


===Model of Phe-tRNA(Phe) in the ribosomal pre-accommodated state revealed by cryo-EM===
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
{{ABSTRACT_PUBMED_19020518}}
 
==About this Structure==
[[3ep2]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EP2 OCA].


==See Also==
==See Also==
*[[Elongation factor|Elongation factor]]
*[[Ribosomal protein L11|Ribosomal protein L11]]
*[[Ribosomal protein L11|Ribosomal protein L11]]
*[[Ribosomal protein S12|Ribosomal protein S12]]
== References ==
 
<references/>
==Reference==
__TOC__
<ref group="xtra">PMID:019020518</ref><references group="xtra"/>
</StructureSection>
[[Category: Escherichia coli k-12]]
[[Category: Escherichia coli k-12]]
[[Category: Agirrezabala, X.]]
[[Category: Agirrezabala, X.]]

Revision as of 13:19, 29 September 2014

Model of Phe-tRNA(Phe) in the ribosomal pre-accommodated state revealed by cryo-EM

3ep2, resolution 9.00Å

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