1guc: Difference between revisions
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[[Image:1guc.gif|left|200px]] | [[Image:1guc.gif|left|200px]] | ||
'''SOLUTION NMR STRUCTURE OF AN RNA WITH TANDEM, SYMMETRIC GU MISMATCHES, 30 STRUCTURES''' | {{Structure | ||
|PDB= 1guc |SIZE=350|CAPTION= <scene name='initialview01'>1guc</scene> | |||
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'''SOLUTION NMR STRUCTURE OF AN RNA WITH TANDEM, SYMMETRIC GU MISMATCHES, 30 STRUCTURES''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1GUC is a [ | 1GUC is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GUC OCA]. | ||
==Reference== | ==Reference== | ||
Investigation of the structural basis for thermodynamic stabilities of tandem GU mismatches: solution structure of (rGAGGUCUC)2 by two-dimensional NMR and simulated annealing., McDowell JA, Turner DH, Biochemistry. 1996 Nov 12;35(45):14077-89. PMID:[http:// | Investigation of the structural basis for thermodynamic stabilities of tandem GU mismatches: solution structure of (rGAGGUCUC)2 by two-dimensional NMR and simulated annealing., McDowell JA, Turner DH, Biochemistry. 1996 Nov 12;35(45):14077-89. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8916893 8916893] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Mcdowell, J A.]] | [[Category: Mcdowell, J A.]] | ||
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[[Category: ribonucleic acid]] | [[Category: ribonucleic acid]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:28:00 2008'' | ||
Revision as of 09:28, 20 March 2008
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SOLUTION NMR STRUCTURE OF AN RNA WITH TANDEM, SYMMETRIC GU MISMATCHES, 30 STRUCTURES
Overview
The duplex (rGAGGUCUC)2 contains the motif [sequence: see text] which is unusually stable compared with other symmetric tandem GU mismatches and occurs in the P5 helix of the group I intron of Tetrahymena thermophila. The three-dimensional solution structure of (rGAGGUCUC)2 was determined using two-dimensional NMR and a simulated annealing protocol. The structure is remarkably similar to the A-DNA crystal structure of (dGGGGTCCC)2 [Kneale, G., Brown, T., & Kennard, O. (1985) J. Mol. Biol. 186, 805-814] which contains the analogous motif [sequence: see text]. Incorporation of the [sequence: see text] motif has little effect on backbone torsion angles and helical parameters compared with standard A-form. The only significant departure from A-form is a slight overtwisting 5' of the G in the GU mismatch and a displacement of the mismatches toward the minor groove. Inspection of stacking patterns of this structure and comparison with symmetric tandem GT mismatches in A-DNA oligonucleotides from crystal structure data suggest that electrostatics are important in determining motif stability.
About this Structure
1GUC is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Investigation of the structural basis for thermodynamic stabilities of tandem GU mismatches: solution structure of (rGAGGUCUC)2 by two-dimensional NMR and simulated annealing., McDowell JA, Turner DH, Biochemistry. 1996 Nov 12;35(45):14077-89. PMID:8916893
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