1hht: Difference between revisions
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[[Image:1hht.gif|left|200px]] | [[Image:1hht.gif|left|200px]] | ||
'''RNA DEPENDENT RNA POLYMERASE FROM DSRNA BACTERIOPHAGE PHI6 PLUS TEMPLATE''' | {{Structure | ||
|PDB= 1hht |SIZE=350|CAPTION= <scene name='initialview01'>1hht</scene>, resolution 2.9Å | |||
|SITE= <scene name='pdbsite=CA1:The+Three+Conserved+Active+Site+Aspartate'>CA1</scene>, <scene name='pdbsite=CA2:The+Three+Conserved+Active+Site+Aspartate'>CA2</scene>, <scene name='pdbsite=CA3:The+Three+Conserved+Active+Site+Aspartate'>CA3</scene>, <scene name='pdbsite=MN1:Mn+Binding+Site+For+Chain+A'>MN1</scene>, <scene name='pdbsite=MN2:Mn+Binding+Site+For+Chain+B'>MN2</scene> and <scene name='pdbsite=MN3:Mn+Binding+Site+For+Chain+C'>MN3</scene> | |||
|LIGAND= <scene name='pdbligand=MN:MANGANESE (II) ION'>MN</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''RNA DEPENDENT RNA POLYMERASE FROM DSRNA BACTERIOPHAGE PHI6 PLUS TEMPLATE''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1HHT is a [ | 1HHT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_phi-6 Bacteriophage phi-6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HHT OCA]. | ||
==Reference== | ==Reference== | ||
A mechanism for initiating RNA-dependent RNA polymerization., Butcher SJ, Grimes JM, Makeyev EV, Bamford DH, Stuart DI, Nature. 2001 Mar 8;410(6825):235-40. PMID:[http:// | A mechanism for initiating RNA-dependent RNA polymerization., Butcher SJ, Grimes JM, Makeyev EV, Bamford DH, Stuart DI, Nature. 2001 Mar 8;410(6825):235-40. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11242087 11242087] | ||
[[Category: Bacteriophage phi-6]] | [[Category: Bacteriophage phi-6]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: viral polymerase]] | [[Category: viral polymerase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:37:17 2008'' | ||
Revision as of 09:37, 20 March 2008
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| 1hht, resolution 2.9Å | |||||||||||||
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| Sites: | CA1, CA2, CA3, MN1, MN2 and MN3 | ||||||||||||
| Ligands: | MN | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
RNA DEPENDENT RNA POLYMERASE FROM DSRNA BACTERIOPHAGE PHI6 PLUS TEMPLATE
Overview
In most RNA viruses, genome replication and transcription are catalysed by a viral RNA-dependent RNA polymerase. Double-stranded RNA viruses perform these operations in a capsid (the polymerase complex), using an enzyme that can read both single- and double-stranded RNA. Structures have been solved for such viral capsids, but they do not resolve the polymerase subunits in any detail. Here we show that the 2 A resolution X-ray structure of the active polymerase subunit from the double-stranded RNA bacteriophage straight phi6 is highly similar to that of the polymerase of hepatitis C virus, providing an evolutionary link between double-stranded RNA viruses and flaviviruses. By crystal soaking and co-crystallization, we determined a number of other structures, including complexes with oligonucleotide and/or nucleoside triphosphates (NTPs), that suggest a mechanism by which the incoming double-stranded RNA is opened up to feed the template through to the active site, while the substrates enter by another route. The template strand initially overshoots, locking into a specificity pocket, and then, in the presence of cognate NTPs, reverses to form the initiation complex; this process engages two NTPs, one of which acts with the carboxy-terminal domain of the protein to prime the reaction. Our results provide a working model for the initiation of replication and transcription.
About this Structure
1HHT is a Single protein structure of sequence from Bacteriophage phi-6. Full crystallographic information is available from OCA.
Reference
A mechanism for initiating RNA-dependent RNA polymerization., Butcher SJ, Grimes JM, Makeyev EV, Bamford DH, Stuart DI, Nature. 2001 Mar 8;410(6825):235-40. PMID:11242087
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