1hl3: Difference between revisions
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[[Image:1hl3.jpg|left|200px]] | [[Image:1hl3.jpg|left|200px]] | ||
'''CTBP/BARS IN TERNARY COMPLEX WITH NAD(H) AND PIDLSKK PEPTIDE''' | {{Structure | ||
|PDB= 1hl3 |SIZE=350|CAPTION= <scene name='initialview01'>1hl3</scene>, resolution 3.1Å | |||
|SITE= <scene name='pdbsite=AC1:Nad+Binding+Site+For+Chain+A'>AC1</scene> | |||
|LIGAND= <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''CTBP/BARS IN TERNARY COMPLEX WITH NAD(H) AND PIDLSKK PEPTIDE''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1HL3 is a [ | 1HL3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HL3 OCA]. | ||
==Reference== | ==Reference== | ||
CtBP/BARS: a dual-function protein involved in transcription co-repression and Golgi membrane fission., Nardini M, Spano S, Cericola C, Pesce A, Massaro A, Millo E, Luini A, Corda D, Bolognesi M, EMBO J. 2003 Jun 16;22(12):3122-30. PMID:[http:// | CtBP/BARS: a dual-function protein involved in transcription co-repression and Golgi membrane fission., Nardini M, Spano S, Cericola C, Pesce A, Massaro A, Millo E, Luini A, Corda D, Bolognesi M, EMBO J. 2003 Jun 16;22(12):3122-30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12805226 12805226] | ||
[[Category: Rattus norvegicus]] | [[Category: Rattus norvegicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: acyl-coa]] | [[Category: acyl-coa]] | ||
[[Category: acyltransferase]] | [[Category: acyltransferase]] | ||
[[Category: brefeldin | [[Category: brefeldin some]] | ||
[[Category: golgi membrane]] | [[Category: golgi membrane]] | ||
[[Category: nad]] | [[Category: nad]] | ||
[[Category: transcription co-repression]] | [[Category: transcription co-repression]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:38:30 2008'' | ||
Revision as of 09:38, 20 March 2008
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| 1hl3, resolution 3.1Å | |||||||||||||
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| Sites: | AC1 | ||||||||||||
| Ligands: | NAD | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
CTBP/BARS IN TERNARY COMPLEX WITH NAD(H) AND PIDLSKK PEPTIDE
Overview
C-terminal-binding protein/brefeldin A-ADP ribosylated substrate (CtBP/BARS) plays key roles in development and oncogenesis as a transcription co-repressor, and in intracellular traffic as a promoter of Golgi membrane fission. Co-repressor activity is regulated by NAD(H) binding to CtBP/BARS, while membrane fission is associated with its acyl-CoA-dependent acyltransferase activity. Here, we report the crystal structures of rat CtBP/BARS in a binary complex with NAD(H), and in a ternary complex with a PIDLSKK peptide mimicking the consensus motif (PXDLS) recognized in CtBP/BARS cellular partners. The structural data show CtBP/BARS in a NAD(H)-bound dimeric form; the peptide binding maps the recognition site for DNA-binding proteins and histone deacetylases to an N-terminal region of the protein. The crystal structure together with the site-directed mutagenesis data and binding experiments suggest a rationale for the molecular mechanisms underlying the two fundamental co-existing, but diverse, activities supported by CtBP/BARS in the nucleus and in Golgi membranes.
About this Structure
1HL3 is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
CtBP/BARS: a dual-function protein involved in transcription co-repression and Golgi membrane fission., Nardini M, Spano S, Cericola C, Pesce A, Massaro A, Millo E, Luini A, Corda D, Bolognesi M, EMBO J. 2003 Jun 16;22(12):3122-30. PMID:12805226
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