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{{Large structure}}
==50S ribosomal subunit from a crystal structure of release factor RF2, tRNAs and mRNA bound to the ribosome. This file contains the 50S subunit from a crystal structure of release factor RF1, tRNAs and mRNA bound to the ribosome and is described in remark 400.==
{{STRUCTURE_2b9n|  PDB=2b9n  |  SCENE=  }}
<StructureSection load='2b9n' size='340' side='right' caption='[[2b9n]], [[Resolution|resolution]] 6.76&Aring;' scene=''>
===50S ribosomal subunit from a crystal structure of release factor RF2, tRNAs and mRNA bound to the ribosome. This file contains the 50S subunit from a crystal structure of release factor RF1, tRNAs and mRNA bound to the ribosome and is described in remark 400.===
== Structural highlights ==
{{ABSTRACT_PUBMED_16377566}}
<table><tr><td colspan='2'>[[2b9n]] is a 30 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B9N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2B9N FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2b64|2b64]], [[2b66|2b66]], [[2b9m|2b9m]], [[2b9o|2b9o]], [[2b9p|2b9p]]</td></tr>
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b9n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b9n OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2b9n RCSB], [http://www.ebi.ac.uk/pdbsum/2b9n PDBsum]</span></td></tr>
<table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b9/2b9n_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
During protein synthesis, translational release factors catalyze the release of the polypeptide chain when a stop codon on the mRNA reaches the A site of the ribosome. The detailed mechanism of this process is currently unknown. We present here the crystal structures of the ribosome from Thermus thermophilus with RF1 and RF2 bound to their cognate stop codons, at resolutions of 5.9 Angstrom and 6.7 Angstrom, respectively. The structures reveal details of interactions of the factors with the ribosome and mRNA, including elements previously implicated in decoding and peptide release. They also shed light on conformational changes both in the factors and in the ribosome during termination. Differences seen in the interaction of RF1 and RF2 with the L11 region of the ribosome allow us to rationalize previous biochemical data. Finally, this work demonstrates the feasibility of crystallizing ribosomes with bound factors at a defined state along the translational pathway.


==About this Structure==
Crystal structures of the ribosome in complex with release factors RF1 and RF2 bound to a cognate stop codon.,Petry S, Brodersen DE, Murphy FV 4th, Dunham CM, Selmer M, Tarry MJ, Kelley AC, Ramakrishnan V Cell. 2005 Dec 29;123(7):1255-66. PMID:16377566<ref>PMID:16377566</ref>
[[2b9n]] is a 30 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B9N OCA].
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>


==See Also==
==See Also==
*[[Ribosomal protein L11|Ribosomal protein L11]]
*[[Ribosome 3D structures|Ribosome 3D structures]]
*[[Ribosomal protein L13|Ribosomal protein L13]]
== References ==
*[[Ribosomal protein L14|Ribosomal protein L14]]
<references/>
*[[Ribosomal protein L15|Ribosomal protein L15]]
__TOC__
*[[Ribosomal protein L16|Ribosomal protein L16]]
</StructureSection>
*[[Ribosomal protein L17|Ribosomal protein L17]]
*[[Ribosomal protein L18|Ribosomal protein L18]]
*[[Ribosomal protein L19|Ribosomal protein L19]]
*[[Ribosomal protein L2|Ribosomal protein L2]]
*[[Ribosomal protein L20|Ribosomal protein L20]]
*[[Ribosomal protein L21|Ribosomal protein L21]]
*[[Ribosomal protein L22|Ribosomal protein L22]]
*[[Ribosomal protein L23|Ribosomal protein L23]]
*[[Ribosomal protein L27|Ribosomal protein L27]]
*[[Ribosomal protein L29|Ribosomal protein L29]]
*[[Ribosomal protein L3|Ribosomal protein L3]]
*[[Ribosomal protein L30|Ribosomal protein L30]]
*[[Ribosomal protein L31|Ribosomal protein L31]]
*[[Ribosomal protein L32|Ribosomal protein L32]]
*[[Ribosomal protein L33|Ribosomal protein L33]]
*[[Ribosomal protein L34|Ribosomal protein L34]]
*[[Ribosomal protein L35|Ribosomal protein L35]]
*[[Ribosomal protein L36|Ribosomal protein L36]]
*[[Ribosomal protein L4|Ribosomal protein L4]]
*[[Ribosomal protein L5|Ribosomal protein L5]]
*[[Ribosomal protein L6|Ribosomal protein L6]]
*[[Ribosomal protein L9|Ribosomal protein L9]]
 
==Reference==
<ref group="xtra">PMID:016377566</ref><references group="xtra"/>
[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
[[Category: Brodersen, D E.]]
[[Category: Brodersen, D E.]]
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