2wza: Difference between revisions

Line 1:

[[Image:~~2wza~~.~~png~~|~~left~~|~~200px~~]]

==TWO INTRAMOLECULAR ISOPEPTIDE BONDS ARE IDENTIFIED IN THE CRYSTAL STRUCTURE OF THE STREPTOCOCCUS GORDONII SSPB C-TERMINAL DOMAIN==

== Structural highlights ==

<table><tr><td colspan='2'>[[2wza]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_gordonii Streptococcus gordonii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WZA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WZA FirstGlance]. <br>

</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene><br>

<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2woy|2woy]], [[2wd6|2wd6]], [[2wqs|2wqs]]</td></tr>

<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wza OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wza RCSB], [http://www.ebi.ac.uk/pdbsum/2wza PDBsum]</span></td></tr>

<table>

== Evolutionary Conservation ==

[[Image:Consurf_key_small.gif|200px|right]]

Check<jmol>

<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wz/2wza_consurf.spt"</scriptWhenChecked>

<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>

<text>to colour the structure by Evolutionary Conservation</text>

</jmolCheckbox>

</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].

== Publication Abstract from PubMed ==

Streptococcus gordonii is a primary colonizer and is involved in the formation of dental plaque. This bacterium expresses several surface proteins. One of them is the adhesin SspB, which is a member of the Antigen I/II family of proteins. SspB is a large multi-domain protein that has interactions with surface molecules on other bacteria and on host cells, and is thus a key factor in the formation of biofilms. Here, we report the crystal structure of a truncated form of the SspB C-terminal domain, solved by single-wavelength anomalous dispersion to 1.5 A resolution. The structure represents the first of a C-terminal domain from a streptococcal Antigen I/II protein and is comprised of two structurally related beta-sandwich domains, C2 and C3, both with a Ca(2+) bound in equivalent positions. In each of the domains, a covalent isopeptide bond is observed between a lysine and an asparagine, a feature that is believed to be a common stabilization mechanism in Gram-positive surface proteins. S. gordonii biofilms contain attachment sites for the periodontal pathogen Porphyromonas gingivalis and the SspB C-terminal domain has been shown to have one such recognition motif, the SspB adherence region. The motif protrudes from the protein, and serves as a handle for attachment. The structure suggests several additional putative binding surfaces, and other binding clefts may be created when the full-length protein is folded.

~~{{STRUCTURE_2wza| PDB=2wza | SCENE= }}~~

Two intramolecular isopeptide bonds are identified in the crystal structure of the Streptococcus gordonii SspB C-terminal domain.,Forsgren N, Lamont RJ, Persson K J Mol Biol. 2010 Apr 2;397(3):740-51. Epub 2010 Feb 4. PMID:20138058<ref>PMID:20138058</ref>

~~===TWO INTRAMOLECULAR ISOPEPTIDE BONDS ARE IDENTIFIED IN THE CRYSTAL STRUCTURE OF THE STREPTOCOCCUS GORDONII SSPB C-TERMINAL DOMAIN===~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

</div>

~~{{ABSTRACT_PUBMED_20138058}}~~

== References ==

~~==About this Structure==~~

__TOC__

~~[[2wza]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Streptococcus_gordonii Streptococcus gordonii]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2WZA OCA].~~

</StructureSection>

==~~Reference~~==

<~~ref group="xtra">PMID:020138058<~~/~~ref~~><~~references group="xtra"~~/>

[[Category: Streptococcus gordonii]]

[[Category: Forsgren, N.]]

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-[[Image:2wza.png|left|200px]]
+==TWO INTRAMOLECULAR ISOPEPTIDE BONDS ARE IDENTIFIED IN THE CRYSTAL STRUCTURE OF THE STREPTOCOCCUS GORDONII SSPB C-TERMINAL DOMAIN==
+<StructureSection load='2wza' size='340' side='right' caption='[[2wza]], [[Resolution|resolution]] 2.08&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2wza]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_gordonii Streptococcus gordonii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WZA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WZA FirstGlance]. <br>
+</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene><br>
+<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2woy|2woy]], [[2wd6|2wd6]], [[2wqs|2wqs]]</td></tr>
+<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wza OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wza RCSB], [http://www.ebi.ac.uk/pdbsum/2wza PDBsum]</span></td></tr>
+<table>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wz/2wza_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Streptococcus gordonii is a primary colonizer and is involved in the formation of dental plaque. This bacterium expresses several surface proteins. One of them is the adhesin SspB, which is a member of the Antigen I/II family of proteins. SspB is a large multi-domain protein that has interactions with surface molecules on other bacteria and on host cells, and is thus a key factor in the formation of biofilms. Here, we report the crystal structure of a truncated form of the SspB C-terminal domain, solved by single-wavelength anomalous dispersion to 1.5 A resolution. The structure represents the first of a C-terminal domain from a streptococcal Antigen I/II protein and is comprised of two structurally related beta-sandwich domains, C2 and C3, both with a Ca(2+) bound in equivalent positions. In each of the domains, a covalent isopeptide bond is observed between a lysine and an asparagine, a feature that is believed to be a common stabilization mechanism in Gram-positive surface proteins. S. gordonii biofilms contain attachment sites for the periodontal pathogen Porphyromonas gingivalis and the SspB C-terminal domain has been shown to have one such recognition motif, the SspB adherence region. The motif protrudes from the protein, and serves as a handle for attachment. The structure suggests several additional putative binding surfaces, and other binding clefts may be created when the full-length protein is folded.
-{{STRUCTURE_2wza|  PDB=2wza  |  SCENE=  }}
+Two intramolecular isopeptide bonds are identified in the crystal structure of the Streptococcus gordonii SspB C-terminal domain.,Forsgren N, Lamont RJ, Persson K J Mol Biol. 2010 Apr 2;397(3):740-51. Epub 2010 Feb 4. PMID:20138058<ref>PMID:20138058</ref>
-===TWO INTRAMOLECULAR ISOPEPTIDE BONDS ARE IDENTIFIED IN THE CRYSTAL STRUCTURE OF THE STREPTOCOCCUS GORDONII SSPB C-TERMINAL DOMAIN===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-{{ABSTRACT_PUBMED_20138058}}
+== References ==
+<references/>
-==About this Structure==
+__TOC__
-[[2wza]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_gordonii Streptococcus gordonii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WZA OCA].
+</StructureSection>
-==Reference==
-<ref group="xtra">PMID:020138058</ref><references group="xtra"/>
 [[Category: Streptococcus gordonii]]
 [[Category: Forsgren, N.]]