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[[Image:2wzl.png|left|200px]]
==THE STRUCTURE OF THE N-RNA BINDING DOMAIN OF THE MOKOLA VIRUS PHOSPHOPROTEIN==
<StructureSection load='2wzl' size='340' side='right' caption='[[2wzl]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2wzl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mokola_virus Mokola virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WZL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WZL FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene><br>
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wzl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wzl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wzl RCSB], [http://www.ebi.ac.uk/pdbsum/2wzl PDBsum]</span></td></tr>
<table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wz/2wzl_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Mokola virus (MOKV) is a nonsegmented, negative-sense RNA virus that belongs to the Lyssavirus genus and Rhabdoviridae family. MOKV phosphoprotein P is an essential component of the replication and transcription complex and acts as a cofactor for the viral RNA-dependent RNA polymerase. P recruits the viral polymerase to the nucleoprotein-bound viral RNA (N-RNA) via an interaction between its C-terminal domain and the N-RNA complex. Here we present a structure for this domain of MOKV P, obtained by expression of full-length P in Escherichia coli, which was subsequently truncated during crystallization. The structure has a high degree of homology with P of rabies virus, another member of Lyssavirus genus, and to a lesser degree with P of vesicular stomatitis virus (VSV), a member of the related Vesiculovirus genus. In addition, analysis of the crystal packing of this domain reveals a potential binding site for the nucleoprotein N. Using both site-directed mutagenesis and yeast two-hybrid experiments to measure P-N interaction, we have determined the relative roles of key amino acids involved in this interaction to map the region of P that binds N. This analysis also reveals a structural relationship between the N-RNA binding domain of the P proteins of the Rhabdoviridae and the Paramyxoviridae.


{{STRUCTURE_2wzl|  PDB=2wzl  |  SCENE=  }}
Structure of the nucleoprotein binding domain of Mokola virus phosphoprotein.,Assenberg R, Delmas O, Ren J, Vidalain PO, Verma A, Larrous F, Graham SC, Tangy F, Grimes JM, Bourhy H J Virol. 2010 Jan;84(2):1089-96. Epub 2009 Nov 11. PMID:19906936<ref>PMID:19906936</ref>


===THE STRUCTURE OF THE N-RNA BINDING DOMAIN OF THE MOKOLA VIRUS PHOSPHOPROTEIN===
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
{{ABSTRACT_PUBMED_19906936}}
== References ==
 
<references/>
==About this Structure==
__TOC__
[[2wzl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mokola_virus Mokola virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WZL OCA].
</StructureSection>
 
==Reference==
<ref group="xtra">PMID:019906936</ref><references group="xtra"/>
[[Category: Mokola virus]]
[[Category: Mokola virus]]
[[Category: Assenberg, R.]]
[[Category: Assenberg, R.]]
Retrieved from "https://proteopedia.org/2wzl"