2ro2: Difference between revisions

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[[~~Image~~:2ro2.~~png|left|200px~~]]

==Solution structure of domain I of the negative polarity CChMVd hammerhead ribozyme==

== Structural highlights ==

<table><tr><td colspan='2'>[[2ro2]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RO2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RO2 FirstGlance]. <br>

</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ro2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ro2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ro2 RCSB], [http://www.ebi.ac.uk/pdbsum/2ro2 PDBsum]</span></td></tr>

<table>

== Publication Abstract from PubMed ==

Loop-loop tertiary interactions play a key role in the folding and catalytic activity of natural hammerhead ribozymes. Using a combination of NMR spectroscopy, site-directed mutagenesis and kinetic and infectivity analyses, we have examined the structure and function of loops 1 and 2 of the (+) and (-) hammerheads of chrysanthemum chlorotic mottle viroid RNA. In both hammerheads, loop 1 is a heptanucleotide hairpin loop containing an exposed U at its 5' side and an extrahelical U at its 3'-side critical for the catalytic activity of the ribozyme in vitro and for viroid infectivity in vivo, whereas loop 2 has a key opened A at its 3'-side. These structural features promote a specific loop-loop interaction motif across the major groove. The essential features of this tertiary structure element, base pairing between the 5' U of loop 1 and the 3' A of loop 2, and interaction of the extrahelical pyrimidine of loop 1 with loop 2, are likely shared by a significant fraction of natural hammerheads.

~~{{STRUCTURE_2ro2| PDB=2ro2 | SCENE= }}~~

Structure-function analysis of the ribozymes of chrysanthemum chlorotic mottle viroid: a loop-loop interaction motif conserved in most natural hammerheads.,Dufour D, de la Pena M, Gago S, Flores R, Gallego J Nucleic Acids Res. 2008 Nov 29. PMID:19043070<ref>PMID:19043070</ref>

~~===Solution structure of domain I~~ of the ~~negative polarity CChMVd hammerhead ribozyme===~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

</div>

~~{{ABSTRACT_PUBMED_19043070}}~~

~~==About this Structure==~~

~~[[2ro2]] is a 1 chain structure~~. ~~Full experimental information is available from [http://oca~~.~~weizmann~~.~~ac.il/oca-bin~~/~~ocashort?id=2RO2 OCA].~~

==See Also==

*[[Ribozyme|Ribozyme]]

== References ==

==~~Reference~~==

<~~ref group="xtra">PMID:019043070<~~/~~ref~~><~~references group="xtra"~~/>

__TOC__

</StructureSection>

[[Category: Dufour, D.]]

[[Category: Flores, R.]]

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@@ Line 1: / Line 1: @@
-[[Image:2ro2.png|left|200px]]
+==Solution structure of domain I of the negative polarity CChMVd hammerhead ribozyme==
+<StructureSection load='2ro2' size='340' side='right' caption='[[2ro2]], [[NMR_Ensembles_of_Models | 1 NMR models]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2ro2]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RO2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RO2 FirstGlance]. <br>
+</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ro2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ro2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ro2 RCSB], [http://www.ebi.ac.uk/pdbsum/2ro2 PDBsum]</span></td></tr>
+<table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Loop-loop tertiary interactions play a key role in the folding and catalytic activity of natural hammerhead ribozymes. Using a combination of NMR spectroscopy, site-directed mutagenesis and kinetic and infectivity analyses, we have examined the structure and function of loops 1 and 2 of the (+) and (-) hammerheads of chrysanthemum chlorotic mottle viroid RNA. In both hammerheads, loop 1 is a heptanucleotide hairpin loop containing an exposed U at its 5' side and an extrahelical U at its 3'-side critical for the catalytic activity of the ribozyme in vitro and for viroid infectivity in vivo, whereas loop 2 has a key opened A at its 3'-side. These structural features promote a specific loop-loop interaction motif across the major groove. The essential features of this tertiary structure element, base pairing between the 5' U of loop 1 and the 3' A of loop 2, and interaction of the extrahelical pyrimidine of loop 1 with loop 2, are likely shared by a significant fraction of natural hammerheads.
-{{STRUCTURE_2ro2|  PDB=2ro2  |  SCENE=  }}
+Structure-function analysis of the ribozymes of chrysanthemum chlorotic mottle viroid: a loop-loop interaction motif conserved in most natural hammerheads.,Dufour D, de la Pena M, Gago S, Flores R, Gallego J Nucleic Acids Res. 2008 Nov 29. PMID:19043070<ref>PMID:19043070</ref>
-===Solution structure of domain I of the negative polarity CChMVd hammerhead ribozyme===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-{{ABSTRACT_PUBMED_19043070}}
-==About this Structure==
-[[2ro2]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RO2 OCA].
 ==See Also==
 *[[Ribozyme|Ribozyme]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:019043070</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
 [[Category: Dufour, D.]]
 [[Category: Flores, R.]]