2psk: Difference between revisions

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~~{{Theoretical_model}}~~

==THEORETICAL MODEL OF AN FAB FRAGMENT COMPLEXED WITH THE MELANOMA-ASSOCIATED GD2 GANGLIOSIDE==

== Structural highlights ==

<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PSK FirstGlance]. <br>

</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2psk FirstGlance], [http://www.ebi.ac.uk/pdbsum/2psk PDBsum]</span></td></tr>

<table>

== Publication Abstract from PubMed ==

The GD2 ganglioside is a cell-surface component that appears on the surface of metastatic melanoma cells and is a marker for the progression of the disease. The ME36.1 monoclonal antibody binds to the GD2 ganglioside and has shown potential as a therapeutic antibody. ME36.1 is a possible alternative therapy to radiation, which is often ineffective in late-stage melanoma. The crystal structure of the Fab fragment of ME36.1 has been determined using molecular replacement and refined to an R factor of 20.4% at 2.8 A resolution. The model has good geometry with root-mean-square deviations of 0.008 A from ideal bond lengths and 1.7 degrees from ideal bond angles. The crystal structure of the ME36.1 Fab shows that its complementarity determining region forms a groove-shaped binding site rather than the pocket-type observed in other sugar binding Fabs. Molecular modeling has placed a four-residue sugar, representative of GD2, in the antigen binding site. The GD2 sugar moiety is stabilized by a network of hydrogen bonds that define the specificity of ME36.1 toward its antigen.

~~[[Image~~:~~2psk~~.~~png|left|200px]]~~

The crystal structure of a Fab fragment to the melanoma-associated GD2 ganglioside.,Pichla SL, Murali R, Burnett RM J Struct Biol. 1997 Jun;119(1):6-16. PMID:9216084<ref>PMID:9216084</ref>

~~{{STRUCTURE_2psk| PDB=2psk | SCENE= }}~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

</div>

~~===THEORETICAL MODEL OF AN FAB FRAGMENT COMPLEXED WITH THE MELANOMA-ASSOCIATED GD2 GANGLIOSIDE===~~

== References ==

~~{{ABSTRACT_PUBMED_9216084}}~~

__TOC__

</StructureSection>

==~~Reference~~==

<~~ref group="xtra">PMID:009216084<~~/~~ref~~><~~references group="xtra"~~/>

[[Category: Burnett, R M]]

[[Category: Murali, R]]

[[Category: Pichla, S L]]

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@@ Line 1: / Line 1: @@
-{{Theoretical_model}}
+==THEORETICAL MODEL OF AN FAB FRAGMENT COMPLEXED WITH THE MELANOMA-ASSOCIATED GD2 GANGLIOSIDE==
+<StructureSection load='2psk' size='340' side='right' caption='[[2psk]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PSK FirstGlance]. <br>
+</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2psk FirstGlance], [http://www.ebi.ac.uk/pdbsum/2psk PDBsum]</span></td></tr>
+<table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The GD2 ganglioside is a cell-surface component that appears on the surface of metastatic melanoma cells and is a marker for the progression of the disease. The ME36.1 monoclonal antibody binds to the GD2 ganglioside and has shown potential as a therapeutic antibody. ME36.1 is a possible alternative therapy to radiation, which is often ineffective in late-stage melanoma. The crystal structure of the Fab fragment of ME36.1 has been determined using molecular replacement and refined to an R factor of 20.4% at 2.8 A resolution. The model has good geometry with root-mean-square deviations of 0.008 A from ideal bond lengths and 1.7 degrees from ideal bond angles. The crystal structure of the ME36.1 Fab shows that its complementarity determining region forms a groove-shaped binding site rather than the pocket-type observed in other sugar binding Fabs. Molecular modeling has placed a four-residue sugar, representative of GD2, in the antigen binding site. The GD2 sugar moiety is stabilized by a network of hydrogen bonds that define the specificity of ME36.1 toward its antigen.
-[[Image:2psk.png|left|200px]]
+The crystal structure of a Fab fragment to the melanoma-associated GD2 ganglioside.,Pichla SL, Murali R, Burnett RM J Struct Biol. 1997 Jun;119(1):6-16. PMID:9216084<ref>PMID:9216084</ref>
-{{STRUCTURE_2psk|  PDB=2psk  |  SCENE=  }}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-===THEORETICAL MODEL OF AN FAB FRAGMENT COMPLEXED WITH THE MELANOMA-ASSOCIATED GD2 GANGLIOSIDE===
+== References ==
+<references/>
-{{ABSTRACT_PUBMED_9216084}}
+__TOC__
+</StructureSection>
-==Reference==
-<ref group="xtra">PMID:009216084</ref><references group="xtra"/>
 [[Category: Burnett, R M]]
 [[Category: Murali, R]]
 [[Category: Pichla, S L]]