3bbx: Difference between revisions

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{{Large structure}}
==The Hsp15 protein fitted into the low resolution Cryo-EM map of the 50S.nc-tRNA.Hsp15 complex==
{{STRUCTURE_3bbx|  PDB=3bbx  |  SCENE=  }}
<StructureSection load='3bbx' size='340' side='right' caption='[[3bbx]], [[Resolution|resolution]] 10.00&Aring;' scene=''>
===The Hsp15 protein fitted into the low resolution Cryo-EM map of the 50S.nc-tRNA.Hsp15 complex===
== Structural highlights ==
{{ABSTRACT_PUBMED_19013177}}
<table><tr><td colspan='2'>[[3bbx]] is a 29 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BBX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BBX FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>
<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2aw4|2aw4]], [[3bbu|3bbu]], [[3bbv|3bbv]]</td></tr>
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bbx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bbx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3bbx RCSB], [http://www.ebi.ac.uk/pdbsum/3bbx PDBsum]</span></td></tr>
<table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bb/3bbx_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
When heat shock prematurely dissociates a translating bacterial ribosome, its 50S subunit is prevented from reinitiating protein synthesis by tRNA covalently linked to the unfinished protein chain that remains threaded through the exit tunnel. Hsp15, a highly upregulated bacterial heat shock protein, reactivates such dead-end complexes. Here, we show with cryo-electron microscopy reconstructions and functional assays that Hsp15 translocates the tRNA moiety from the A site to the P site of stalled 50S subunits. By stabilizing the tRNA in the P site, Hsp15 indirectly frees up the A site, allowing a release factor to land there and cleave off the tRNA. Such a release factor must be stop codon independent, suggesting a possible role for a poorly characterized class of putative release factors that are upregulated by cellular stress, lack a codon recognition domain and are conserved in eukaryotes.


==About this Structure==
Recycling of aborted ribosomal 50S subunit-nascent chain-tRNA complexes by the heat shock protein Hsp15.,Jiang L, Schaffitzel C, Bingel-Erlenmeyer R, Ban N, Korber P, Koning RI, de Geus DC, Plaisier JR, Abrahams JP J Mol Biol. 2009 Mar 13;386(5):1357-67. Epub 2008 Nov 5. PMID:19013177<ref>PMID:19013177</ref>
[[3bbx]] is a 29 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BBX OCA].


==See Also==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
*[[Ribosomal protein L13|Ribosomal protein L13]]
</div>
*[[Ribosomal protein L14|Ribosomal protein L14]]
== References ==
*[[Ribosomal protein L15|Ribosomal protein L15]]
<references/>
*[[Ribosomal protein L16|Ribosomal protein L16]]
__TOC__
*[[Ribosomal protein L17|Ribosomal protein L17]]
</StructureSection>
*[[Ribosomal protein L18|Ribosomal protein L18]]
*[[Ribosomal protein L19|Ribosomal protein L19]]
*[[Ribosomal protein L2|Ribosomal protein L2]]
*[[Ribosomal protein L20|Ribosomal protein L20]]
*[[Ribosomal protein L21|Ribosomal protein L21]]
*[[Ribosomal protein L22|Ribosomal protein L22]]
*[[Ribosomal protein L23|Ribosomal protein L23]]
*[[Ribosomal protein L24|Ribosomal protein L24]]
*[[Ribosomal protein L25|Ribosomal protein L25]]
*[[Ribosomal protein L27|Ribosomal protein L27]]
*[[Ribosomal protein L29|Ribosomal protein L29]]
*[[Ribosomal protein L3|Ribosomal protein L3]]
*[[Ribosomal protein L30|Ribosomal protein L30]]
*[[Ribosomal protein L31|Ribosomal protein L31]]
*[[Ribosomal protein L32|Ribosomal protein L32]]
*[[Ribosomal protein L33|Ribosomal protein L33]]
*[[Ribosomal protein L34|Ribosomal protein L34]]
*[[Ribosomal protein L35|Ribosomal protein L35]]
*[[Ribosomal protein L36|Ribosomal protein L36]]
*[[Ribosomal protein L4|Ribosomal protein L4]]
*[[Ribosomal protein L5|Ribosomal protein L5]]
*[[Ribosomal protein L6|Ribosomal protein L6]]
*[[Ribosomal protein L9|Ribosomal protein L9]]
 
==Reference==
<ref group="xtra">PMID:019013177</ref><references group="xtra"/>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Abrahams, J P.]]
[[Category: Abrahams, J P.]]

Revision as of 21:03, 2 October 2014

The Hsp15 protein fitted into the low resolution Cryo-EM map of the 50S.nc-tRNA.Hsp15 complex

3bbx, resolution 10.00Å

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