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{{STRUCTURE_1ema| PDB=1ema | SCENE= }}  
==GREEN FLUORESCENT PROTEIN FROM AEQUOREA VICTORIA==
===GREEN FLUORESCENT PROTEIN FROM AEQUOREA VICTORIA===
<StructureSection load='1ema' size='340' side='right' caption='[[1ema]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
{{ABSTRACT_PUBMED_8703075}}
== Structural highlights ==
<table><tr><td colspan='2'>[[1ema]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. The June 2003 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Green Fluorescent Protein (GFP)'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2003_6 10.2210/rcsb_pdb/mom_2003_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EMA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EMA FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ema FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ema OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ema RCSB], [http://www.ebi.ac.uk/pdbsum/1ema PDBsum]</span></td></tr>
<table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/em/1ema_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The green fluorescent protein (GFP) from the Pacific Northwest jellyfish Aequorea victoria has generated intense interest as a marker for gene expression and localization of gene products. The chromophore, resulting from the spontaneous cyclization and oxidation of the sequence -Ser65 (or Thr65)-Tyr66-Gly67-, requires the native protein fold for both formation and fluorescence emission. The structure of Thr65 GFP has been determined at 1.9 angstrom resolution. The protein fold consists of an 11-stranded beta barrel with a coaxial helix, with the chromophore forming from the central helix. Directed mutagenesis of one residue adjacent to the chromophore, Thr203, to Tyr or His results in significantly red-shifted excitation and emission maxima.


==About this Structure==
Crystal structure of the Aequorea victoria green fluorescent protein.,Ormo M, Cubitt AB, Kallio K, Gross LA, Tsien RY, Remington SJ Science. 1996 Sep 6;273(5280):1392-5. PMID:8703075<ref>PMID:8703075</ref>
[[1ema]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. The June 2003 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Green Fluorescent Protein (GFP)''  by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2003_6 10.2210/rcsb_pdb/mom_2003_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EMA OCA].
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>


==See Also==
==See Also==
*[[Alyssa Marsico/Sandbox 1|Alyssa Marsico/Sandbox 1]]
*[[Devon McCarthy/Sandbox 1|Devon McCarthy/Sandbox 1]]
*[[Gfp vc2|Gfp vc2]]
*[[Gfp vc2|Gfp vc2]]
*[[Green Fluorescent Protein|Green Fluorescent Protein]]
*[[Green Fluorescent Protein|Green Fluorescent Protein]]
*[[Green Fluorescent Protein: Research Tool|Green Fluorescent Protein: Research Tool]]
*[[Green Fluorescent Protein: Research Tool|Green Fluorescent Protein: Research Tool]]
*[[Sandbox104|Sandbox104]]
*[[Proteopedia.org/wiki/index.php/User:Joel L. Sussman/GFP Test Page|Proteopedia.org/wiki/index.php/User:Joel L. Sussman/GFP Test Page]]
*[[User:Joanne Lau/Sandbox 5|User:Joanne Lau/Sandbox 5]]
*[[Proteopedia.org/wiki/workshop/GFP Background|Proteopedia.org/wiki/workshop/GFP Background]]
*[[User:Joel L. Sussman/GFP Test Page|User:Joel L. Sussman/GFP Test Page]]
*[[User:Lynmarie K Thompson/2009Chem490|User:Lynmarie K Thompson/2009Chem490]]
*[[User:Lynmarie K Thompson/2009Chem490|User:Lynmarie K Thompson/2009Chem490]]
*[[User:Student|User:Student]]
== References ==
 
<references/>
==Reference==
__TOC__
<ref group="xtra">PMID:008703075</ref><references group="xtra"/>
</StructureSection>
[[Category: Aequorea victoria]]
[[Category: Aequorea victoria]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: RCSB PDB Molecule of the Month]]

Revision as of 05:33, 3 October 2014

GREEN FLUORESCENT PROTEIN FROM AEQUOREA VICTORIA

1ema, resolution 1.90Å

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