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[[Image:2q57.png|left|200px]]
==X-ray structure of Cerulean GFP: A tryptophan-based chromophore useful for fluorescence lifetime imaging==
<StructureSection load='2q57' size='340' side='right' caption='[[2q57]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2q57]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q57 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2Q57 FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRF:[(4Z)-2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(1H-INDOL-3-YLMETHYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>CRF</scene></td></tr>
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2q57 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q57 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2q57 RCSB], [http://www.ebi.ac.uk/pdbsum/2q57 PDBsum]</span></td></tr>
<table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q5/2q57_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The crystal structure of the cyan-fluorescent Cerulean green fluorescent protein (GFP), a variant of enhanced cyan fluorescent protein (ECFP), has been determined to 2.0 A. Cerulean bears an internal fluorophore composed of an indole moiety derived from Y66W, conjugated to the GFP-like imidazolinone ring via a methylene bridge. Cerulean undergoes highly efficient fluorescence resonance energy transfer (FRET) to yellow acceptor molecules and exhibits significantly reduced excited-state heterogeneity. This feature was rationally engineered in ECFP by substituting His148 with an aspartic acid [Rizzo et al. (2004) Nat. Biotechnol. 22, 445], rendering Cerulean useful for fluorescence lifetime imaging microscopy (FLIM). The X-ray structure is consistent with a single conformation of the chromophore and surrounding residues and may therefore provide a structural rationale for the previously described monoexponential fluorescence decay. Unexpectedly, the carboxyl group of H148D is found in a buried position, directly contacting the indole nitrogen of the chromophore via a bifurcated hydrogen bond. Compared to the similarly constructed ECFP chromophore, the indole group of Cerulean is rotated around the methylene bridge to adopt a cis-coplanar conformation with respect to the imidazolinone ring, resulting in a close edge-to-edge contact of the two ring systems. The double-humped absorbance spectrum persists in single-crystal absorbance measurements, casting doubt on the idea that ground state conformational heterogeneity forms the basis of the two overlapping transitions. At low pH, a blue shift in absorbance of 10-15 nm suggests a pH-induced structural transition that proceeds with a time constant of 47 (+/-2) min and is reversible. Possible interpretations in terms of chromophore isomerization are presented.


{{STRUCTURE_2q57|  PDB=2q57  |  SCENE=  }}
X-ray structure of Cerulean GFP: a tryptophan-based chromophore useful for fluorescence lifetime imaging.,Malo GD, Pouwels LJ, Wang M, Weichsel A, Montfort WR, Rizzo MA, Piston DW, Wachter RM Biochemistry. 2007 Sep 4;46(35):9865-73. Epub 2007 Aug 8. PMID:17685554<ref>PMID:17685554</ref>


===X-ray structure of Cerulean GFP: A tryptophan-based chromophore useful for fluorescence lifetime imaging===
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
{{ABSTRACT_PUBMED_17685554}}
 
==About this Structure==
[[2q57]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q57 OCA].


==See Also==
==See Also==
*[[Alyssa Marsico/Sandbox 1|Alyssa Marsico/Sandbox 1]]
*[[Devon McCarthy/Sandbox 1|Devon McCarthy/Sandbox 1]]
*[[Green Fluorescent Protein|Green Fluorescent Protein]]
*[[Green Fluorescent Protein|Green Fluorescent Protein]]
*[[Sandbox104|Sandbox104]]
== References ==
*[[User:Joanne Lau/Sandbox 5|User:Joanne Lau/Sandbox 5]]
<references/>
 
__TOC__
==Reference==
</StructureSection>
<ref group="xtra">PMID:017685554</ref><references group="xtra"/>
[[Category: Aequorea victoria]]
[[Category: Aequorea victoria]]
[[Category: Malo, G D.]]
[[Category: Malo, G D.]]
[[Category: Beta barrel]]
[[Category: Beta barrel]]
[[Category: Fluorescent protein]]
[[Category: Fluorescent protein]]
Retrieved from "https://proteopedia.org/2q57"