1wlk: Difference between revisions

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[[Image:1wlk.png|left|200px]]
==L122E mutant of FMN-binding protein from Desulfovibrio vulgaris (Miyazaki F)==
<StructureSection load='1wlk' size='340' side='right' caption='[[1wlk]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1wlk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_vulgaris_str._'miyazaki_f' Desulfovibrio vulgaris str. 'miyazaki f']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WLK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WLK FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene><br>
<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1flm|1flm]], [[1wli|1wli]], [[1wll|1wll]]</td></tr>
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wlk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wlk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1wlk RCSB], [http://www.ebi.ac.uk/pdbsum/1wlk PDBsum]</span></td></tr>
<table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wl/1wlk_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Mutants of flavin mononucleotide-binding protein (FMN-bp) were made by site-directed mutagenesis to investigate the role of carboxyl-terminal Leu122 of the pairing subunit in controlling redox potentials, binding the prosthetic group, and forming the tertiary and quaternary structure. We compared the oxidation-reduction potentials, FMN-binding properties, and higher structures of wild-type FMN-bp and four mutant proteins (L122Y, L122E, L122K and L122-deleted). We found that the redox potentials were affected by mutations. Also, the affinities of L122E, L122K and L122 deletion mutant apoproteins for FMN were lower than for the wild-type apoprotein, whereas the affinity of L122Y for FMN was increased. Analytical ultracentrifugation showed that the dissociation constants for dimerization of L122E and L122K were larger than for wild-type FMN-bp, whereas the dissociation constants for L122Y and the deletion mutant were lower than for the wild type. Finally, we determined the higher structures of L122Y, L122E and L122K mutants by X-ray crystallography. Our results show that the mutation of Leu122 in FMN-bp changes midpoint potentials, dissociation constants for FMN, and dimer formation, indicating that this residue is important in the pairing subunit.


{{STRUCTURE_1wlk|  PDB=1wlk  |  SCENE=  }}
Determination of the role of the Carboxyl-terminal leucine-122 in FMN-binding protein by mutational and structural analysis.,Kitamura M, Terakawa K, Inoue H, Hayashida T, Suto K, Morimoto Y, Yasuoka N, Shibata N, Higuchi Y J Biochem. 2007 Apr;141(4):459-68. Epub 2007 Jan 29. PMID:17261542<ref>PMID:17261542</ref>


===L122E mutant of FMN-binding protein from Desulfovibrio vulgaris (Miyazaki F)===
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
{{ABSTRACT_PUBMED_17261542}}
== References ==
 
<references/>
==About this Structure==
__TOC__
[[1wlk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_vulgaris_str._'miyazaki_f' Desulfovibrio vulgaris str. 'miyazaki f']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WLK OCA].
</StructureSection>
 
==Reference==
<ref group="xtra">PMID:017261542</ref><references group="xtra"/>
[[Category: Desulfovibrio vulgaris str. 'miyazaki f']]
[[Category: Desulfovibrio vulgaris str. 'miyazaki f']]
[[Category: Higuchi, Y.]]
[[Category: Higuchi, Y.]]
Retrieved from "https://proteopedia.org/1wlk"