1jba: Difference between revisions
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[[Image:1jba.jpg|left|200px]]< | [[Image:1jba.jpg|left|200px]] | ||
'''UNMYRISTOYLATED GCAP-2 WITH THREE CALCIUM IONS BOUND''' | {{Structure | ||
|PDB= 1jba |SIZE=350|CAPTION= <scene name='initialview01'>1jba</scene> | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Guanylate_cyclase Guanylate cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.2 4.6.1.2] | |||
|GENE= | |||
}} | |||
'''UNMYRISTOYLATED GCAP-2 WITH THREE CALCIUM IONS BOUND''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1JBA is a [ | 1JBA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JBA OCA]. | ||
==Reference== | ==Reference== | ||
Three-dimensional structure of guanylyl cyclase activating protein-2, a calcium-sensitive modulator of photoreceptor guanylyl cyclases., Ames JB, Dizhoor AM, Ikura M, Palczewski K, Stryer L, J Biol Chem. 1999 Jul 2;274(27):19329-37. PMID:[http:// | Three-dimensional structure of guanylyl cyclase activating protein-2, a calcium-sensitive modulator of photoreceptor guanylyl cyclases., Ames JB, Dizhoor AM, Ikura M, Palczewski K, Stryer L, J Biol Chem. 1999 Jul 2;274(27):19329-37. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10383444 10383444] | ||
[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Guanylate cyclase]] | [[Category: Guanylate cyclase]] | ||
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[[Category: guanylyl cyclase regulation]] | [[Category: guanylyl cyclase regulation]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:01:04 2008'' | ||
Revision as of 10:01, 20 March 2008
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| Ligands: | CA | ||||||||||||
| Activity: | Guanylate cyclase, with EC number 4.6.1.2 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
UNMYRISTOYLATED GCAP-2 WITH THREE CALCIUM IONS BOUND
Overview
Guanylyl cyclase activating protein-2 (GCAP-2) is a Ca2+-sensitive regulator of phototransduction in retinal photoreceptor cells. GCAP-2 activates retinal guanylyl cyclases at low Ca2+ concentration (<100 nM) and inhibits them at high Ca2+ (>500 nM). The light-induced lowering of the Ca2+ level from approximately 500 nM in the dark to approximately 50 nM following illumination is known to play a key role in visual recovery and adaptation. We report here the three-dimensional structure of unmyristoylated GCAP-2 with three bound Ca2+ ions as determined by nuclear magnetic resonance spectroscopy of recombinant, isotopically labeled protein. GCAP-2 contains four EF-hand motifs arranged in a compact tandem array like that seen previously in recoverin. The root mean square deviation of the main chain atoms in the EF-hand regions is 2.2 A in comparing the Ca2+-bound structures of GCAP-2 and recoverin. EF-1, as in recoverin, does not bind calcium because it contains a disabling Cys-Pro sequence. GCAP-2 differs from recoverin in that the calcium ion binds to EF-4 in addition to EF-2 and EF-3. A prominent exposed patch of hydrophobic residues formed by EF-1 and EF-2 (Leu24, Trp27, Phe31, Phe45, Phe48, Phe49, Tyr81, Val82, Leu85, and Leu89) may serve as a target-binding site for the transmission of calcium signals to guanylyl cyclase.
About this Structure
1JBA is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
Three-dimensional structure of guanylyl cyclase activating protein-2, a calcium-sensitive modulator of photoreceptor guanylyl cyclases., Ames JB, Dizhoor AM, Ikura M, Palczewski K, Stryer L, J Biol Chem. 1999 Jul 2;274(27):19329-37. PMID:10383444
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