1jb7: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1jb7.gif|left|200px]] | [[Image:1jb7.gif|left|200px]] | ||
'''DNA G-Quartets in a 1.86 A Resolution Structure of an Oxytricha nova Telomeric Protein-DNA Complex''' | {{Structure | ||
|PDB= 1jb7 |SIZE=350|CAPTION= <scene name='initialview01'>1jb7</scene>, resolution 1.86Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=NA:SODIUM+ION'>NA</scene> and <scene name='pdbligand=CL:CHLORIDE ION'>CL</scene> | |||
|ACTIVITY= | |||
|GENE= MAC-56A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=200597 Sterkiella nova]), MAC-41A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=200597 Sterkiella nova]) | |||
}} | |||
'''DNA G-Quartets in a 1.86 A Resolution Structure of an Oxytricha nova Telomeric Protein-DNA Complex''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
1JB7 is a [ | 1JB7 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Sterkiella_nova Sterkiella nova]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JB7 OCA]. | ||
==Reference== | ==Reference== | ||
DNA G-quartets in a 1.86 A resolution structure of an Oxytricha nova telomeric protein-DNA complex., Horvath MP, Schultz SC, J Mol Biol. 2001 Jul 6;310(2):367-77. PMID:[http:// | DNA G-quartets in a 1.86 A resolution structure of an Oxytricha nova telomeric protein-DNA complex., Horvath MP, Schultz SC, J Mol Biol. 2001 Jul 6;310(2):367-77. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11428895 11428895] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Sterkiella nova]] | [[Category: Sterkiella nova]] | ||
| Line 18: | Line 27: | ||
[[Category: NA]] | [[Category: NA]] | ||
[[Category: dna hydration]] | [[Category: dna hydration]] | ||
[[Category: dna-protein | [[Category: dna-protein interaction]] | ||
[[Category: quadruplex dna]] | [[Category: quadruplex dna]] | ||
[[Category: sodium ion]] | [[Category: sodium ion]] | ||
[[Category: telomere-binding protein]] | [[Category: telomere-binding protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:01:05 2008'' | ||
Revision as of 10:01, 20 March 2008
| |||||||||||||
| 1jb7, resolution 1.86Å | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ligands: | NA and CL | ||||||||||||
| Gene: | MAC-56A (Sterkiella nova), MAC-41A (Sterkiella nova) | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
DNA G-Quartets in a 1.86 A Resolution Structure of an Oxytricha nova Telomeric Protein-DNA Complex
Overview
The Oxytricha nova telomere end binding protein (OnTEBP) recognizes, binds and protects the single-stranded 3'-terminal DNA extension found at the ends of macronuclear chromosomes. The structure of this complex shows that the single strand GGGGTTTTGGGG DNA binds in a deep cleft between the two protein subunits of OnTEBP, adopting a non-helical and irregular conformation. In extending the resolution limit of this structure to 1.86 A, we were surprised to find a G-quartet linked dimer of the GGGGTTTTGGGG DNA also packing within the crystal lattice and interacting with the telomere end binding protein. The G-quartet DNA exhibits the same structure and topology as previously observed in solution by NMR with diagonally crossing d(TTTT) loops at either end of the four-stranded helix. Additionally, the crystal structure reveals clearly visible Na(+), and specific patterns of bound water molecules in the four non-equivalent grooves. Although the G-quartet:protein contact surfaces are modest and might simply represent crystal packing interactions, it is interesting to speculate that the two types of telomeric DNA-protein interactions observed here might both be important in telomere biology.
About this Structure
1JB7 is a Protein complex structure of sequences from Sterkiella nova. Full crystallographic information is available from OCA.
Reference
DNA G-quartets in a 1.86 A resolution structure of an Oxytricha nova telomeric protein-DNA complex., Horvath MP, Schultz SC, J Mol Biol. 2001 Jul 6;310(2):367-77. PMID:11428895
Page seeded by OCA on Thu Mar 20 12:01:05 2008
