1wvl: Difference between revisions

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[[~~Image~~:~~1wvl~~.~~png~~|~~left~~|~~200px~~]]

==Crystal Structure of Multimeric DNA-binding Protein Sac7d-GCN4 with DNA decamer==

== Structural highlights ==

<table><tr><td colspan='2'>[[1wvl]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WVL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WVL FirstGlance]. <br>

</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1azp|1azp]], [[1wd0|1wd0]]</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wvl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wvl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1wvl RCSB], [http://www.ebi.ac.uk/pdbsum/1wvl PDBsum]</span></td></tr>

</table>

== Publication Abstract from PubMed ==

The protein Sac7d belongs to a class of small chromosomal proteins from the hyperthermophilic archaeon Sulfolobus acidocaldarius. Sac7d is extremely stable to heat, acid, and chemical agents. This protein is a monomer and it binds DNA without any particular sequence preference, while inducing a sharp kink in the DNA. By appending a leucine-zipper-like helical peptide derived from the yeast transcriptional activator GCN4 to the C-terminal end of Sac7d, the modified monomers (denoted S7dLZ) are expected to interact with each other via hydrophobic force to form a parallel dimer. The recombinant S7dLZ was expressed in Escherichia coli and purified by heating and ion-exchange chromatography. The formation of dimer was detected by gel-filtration chromatography and chemical cross-link. The results of surface plasmon resonance and circular dichroism experiments showed that the DNA-binding capacity was retained. Furthermore, X-ray diffraction analysis of single crystals of S7dLZ in complex with DNA decamer CCTATATAGG showed that the leucine-zipper segments of S7dLZ were associated into an antiparallel four-helix bundle. There are two DNA fragments bound to each S7dLZ tetramer in the crystal. This model works as a successful template that endows protein a new function without losing original properties.

~~{{STRUCTURE_1wvl| PDB=1wvl | SCENE= }}~~

Design and characterization of a multimeric DNA binding protein using Sac7d and GCN4 as templates.,Wu SW, Ko TP, Chou CC, Wang AH Proteins. 2005 Sep 1;60(4):617-28. PMID:16028219<ref>PMID:16028219</ref>

~~===Crystal Structure~~ of ~~Multimeric DNA-binding Protein Sac7d-GCN4 with DNA decamer===~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

</div>

~~{{ABSTRACT_PUBMED_16028219}}~~

==See Also==

*[[Gcn4|Gcn4]]

==~~About this Structure~~==

== References ==

[[~~1wvl~~]] ~~is a 4 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WVL OCA].~~

__TOC__

</StructureSection>

[[Category: Sulfolobus acidocaldarius]]

[[Category: Wang, A H.]]

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-[[Image:1wvl.png|left|200px]]
+==Crystal Structure of Multimeric DNA-binding Protein Sac7d-GCN4 with DNA decamer==
+<StructureSection load='1wvl' size='340' side='right' caption='[[1wvl]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1wvl]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WVL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WVL FirstGlance]. <br>
+</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1azp|1azp]], [[1wd0|1wd0]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wvl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wvl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1wvl RCSB], [http://www.ebi.ac.uk/pdbsum/1wvl PDBsum]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The protein Sac7d belongs to a class of small chromosomal proteins from the hyperthermophilic archaeon Sulfolobus acidocaldarius. Sac7d is extremely stable to heat, acid, and chemical agents. This protein is a monomer and it binds DNA without any particular sequence preference, while inducing a sharp kink in the DNA. By appending a leucine-zipper-like helical peptide derived from the yeast transcriptional activator GCN4 to the C-terminal end of Sac7d, the modified monomers (denoted S7dLZ) are expected to interact with each other via hydrophobic force to form a parallel dimer. The recombinant S7dLZ was expressed in Escherichia coli and purified by heating and ion-exchange chromatography. The formation of dimer was detected by gel-filtration chromatography and chemical cross-link. The results of surface plasmon resonance and circular dichroism experiments showed that the DNA-binding capacity was retained. Furthermore, X-ray diffraction analysis of single crystals of S7dLZ in complex with DNA decamer CCTATATAGG showed that the leucine-zipper segments of S7dLZ were associated into an antiparallel four-helix bundle. There are two DNA fragments bound to each S7dLZ tetramer in the crystal. This model works as a successful template that endows protein a new function without losing original properties.
-{{STRUCTURE_1wvl|  PDB=1wvl  |  SCENE=  }}
+Design and characterization of a multimeric DNA binding protein using Sac7d and GCN4 as templates.,Wu SW, Ko TP, Chou CC, Wang AH Proteins. 2005 Sep 1;60(4):617-28. PMID:16028219<ref>PMID:16028219</ref>
-===Crystal Structure of Multimeric DNA-binding Protein Sac7d-GCN4 with DNA decamer===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-{{ABSTRACT_PUBMED_16028219}}
+==See Also==
+*[[Gcn4|Gcn4]]
-==About this Structure==
+== References ==
-[[1wvl]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WVL OCA].
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Sulfolobus acidocaldarius]]
 [[Category: Wang, A H.]]