1s23: Difference between revisions
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[[ | ==Crystal Structure Analysis of the B-DNA Decamer CGCAATTGCG== | ||
<StructureSection load='1s23' size='340' side='right' caption='[[1s23]], [[Resolution|resolution]] 1.60Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1s23]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S23 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1S23 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s23 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s23 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1s23 RCSB], [http://www.ebi.ac.uk/pdbsum/1s23 PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The deoxyoligonucleotide d(CGCAATTGCG) has previously been crystallized in four different space groups. The crystals diffract to moderate resolution (2.3-2.9 A). Here, a fifth crystal form that diffracts to higher resolution (1.6 A) is presented which was obtained thanks to the use of Co2+ and cryogenic temperatures. The availability of five different crystal structures allows a thorough analysis of the conformational variability of this DNA sequence. It is concluded that the central hexamer sequence CAATTG has a practically constant conformation under all conditions, whilst the terminal base pairs at both ends vary considerably as a result of differing interactions in the crystals. The new crystal structure presented here is stabilized by guanine-Co2+-guanine interactions and the formation of C1+ -G8.C3 triplexes between neighbouring duplexes. As a result of the higher resolution of the crystal structure, a more regular structure was obtained and a clear definition of the spine of hydration was observed which was not visible in the four previous structures. | |||
DNA variability in five crystal structures of d(CGCAATTGCG).,Valls N, Wright G, Steiner RA, Murshudov GN, Subirana JA Acta Crystallogr D Biol Crystallogr. 2004 Apr;60(Pt 4):680-5. Epub 2004, Mar 23. PMID:15039556<ref>PMID:15039556</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
== | __TOC__ | ||
</StructureSection> | |||
[[Category: Murshudov, G N.]] | [[Category: Murshudov, G N.]] | ||
[[Category: Steiner, R A.]] | [[Category: Steiner, R A.]] | ||
Revision as of 08:29, 8 October 2014
Crystal Structure Analysis of the B-DNA Decamer CGCAATTGCG
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