2akg: Difference between revisions

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[[~~Image~~:2akg.~~png|left|200px~~]]

==Thallium form of the G-Quadruplex from Oxytricha Nova, d(G4T4G4)2==

== Structural highlights ==

<table><tr><td colspan='2'>[[2akg]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AKG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AKG FirstGlance]. <br>

</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2akg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2akg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2akg RCSB], [http://www.ebi.ac.uk/pdbsum/2akg PDBsum]</span></td></tr>

</table>

== Publication Abstract from PubMed ==

Monovalent cations play an important role in many biological functions. The guanine rich sequence, d(G4T4G4), requires monovalent cations for formation of the G-quadruplex, d(G4T4G4)2. This requirement can be satisfied by thallium (Tl+), a potassium (K+) surrogate. To verify that the structure of d(G4T4G4)2 in the presence of Tl+ is similar to the K+-form of the G-quadruplex, the solution structure of the Tl+-form of d(G4T4G4)2 was determined. The 10 lowest energy structures have an all atom RMSD of 0.76 +/- 0.16 A. Comparison of this structure to the identical G-quadruplex formed in the presence of K+ validates the isomorphous nature of Tl+ and K+. Using a 1H-205Tl spin-echo difference experiment we show that, in the Tl+-form of d(G4T4G4)2, small scalar couplings (<1 Hz) exist between 205Tl and protons in the G-quadruplex. These data comprise the first 1H-205Tl scalar couplings observed in a biological system and have the potential to provide important constraints for structure determination. These experiments can be applied to any system in which the substituted Tl+ cations are in slow exchange with the bulk ions in solution.

~~{{STRUCTURE_2akg| PDB=2akg | SCENE= }}~~

205Tl NMR methods for the characterization of monovalent cation binding to nucleic acids.,Gill ML, Strobel SA, Loria JP J Am Chem Soc. 2005 Nov 30;127(47):16723-32. PMID:16305263<ref>PMID:16305263</ref>

~~===Thallium form~~ of the ~~G-Quadruplex from Oxytricha Nova, d(G4T4G4)2===~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

</div>

~~{{ABSTRACT_PUBMED_16305263}}~~

== References ==

==~~About this Structure~~==

__TOC__

~~[[2akg]] is a 2 chain structure. Full experimental information is available from [http:~~//~~oca.weizmann.ac.il/oca-bin/ocashort?id=2AKG OCA].~~

</StructureSection>

[[Category: Gill, M L.]]

[[Category: Loria, J P.]]

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@@ Line 1: / Line 1: @@
-[[Image:2akg.png|left|200px]]
+==Thallium form of the G-Quadruplex from Oxytricha Nova, d(G4T4G4)2==
+<StructureSection load='2akg' size='340' side='right' caption='[[2akg]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2akg]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AKG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AKG FirstGlance]. <br>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2akg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2akg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2akg RCSB], [http://www.ebi.ac.uk/pdbsum/2akg PDBsum]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Monovalent cations play an important role in many biological functions. The guanine rich sequence, d(G4T4G4), requires monovalent cations for formation of the G-quadruplex, d(G4T4G4)2. This requirement can be satisfied by thallium (Tl+), a potassium (K+) surrogate. To verify that the structure of d(G4T4G4)2 in the presence of Tl+ is similar to the K+-form of the G-quadruplex, the solution structure of the Tl+-form of d(G4T4G4)2 was determined. The 10 lowest energy structures have an all atom RMSD of 0.76 +/- 0.16 A. Comparison of this structure to the identical G-quadruplex formed in the presence of K+ validates the isomorphous nature of Tl+ and K+. Using a 1H-205Tl spin-echo difference experiment we show that, in the Tl+-form of d(G4T4G4)2, small scalar couplings (&lt;1 Hz) exist between 205Tl and protons in the G-quadruplex. These data comprise the first 1H-205Tl scalar couplings observed in a biological system and have the potential to provide important constraints for structure determination. These experiments can be applied to any system in which the substituted Tl+ cations are in slow exchange with the bulk ions in solution.
-{{STRUCTURE_2akg|  PDB=2akg  |  SCENE=  }}
+Tl NMR methods for the characterization of monovalent cation binding to nucleic acids.,Gill ML, Strobel SA, Loria JP J Am Chem Soc. 2005 Nov 30;127(47):16723-32. PMID:16305263<ref>PMID:16305263</ref>
-===Thallium form of the G-Quadruplex from Oxytricha Nova, d(G4T4G4)2===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-{{ABSTRACT_PUBMED_16305263}}
+== References ==
+<references/>
-==About this Structure==
+__TOC__
-[[2akg]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AKG OCA].
+</StructureSection>
 [[Category: Gill, M L.]]
 [[Category: Loria, J P.]]