2kzd: Difference between revisions
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[[ | ==Structure of a (3+1) G-quadruplex formed by hTERT promoter sequence== | ||
<StructureSection load='2kzd' size='340' side='right' caption='[[2kzd]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2kzd]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KZD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KZD FirstGlance]. <br> | |||
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DI:2-DEOXYINOSINE-5-MONOPHOSPHATE'>DI</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2kze|2kze]], [[2a5p|2a5p]], [[2o3m|2o3m]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kzd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kzd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kzd RCSB], [http://www.ebi.ac.uk/pdbsum/2kzd PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The catalytic subunit of human telomerase, hTERT, actively elongates the 3' end of the telomere in most cancer cells. The hTERT promoter, which contains many guanine-rich stretches on the same DNA strand, exhibits an exceptional potential for G-quadruplex formation. Here we show that one particular G-rich sequence in this region coexists in two G-quadruplex conformations in potassium solution: a (3 + 1) and a parallel-stranded G-quadruplexes. We present the NMR solution structures of both conformations, each comprising several robust structural elements, among which include the (3 + 1) and all-parallel G-tetrad cores, single-residue double-chain-reversal loops, and a capping A.T base pair. A combination of NMR and CD techniques, complemented with sequence modifications and variations of experimental condition, allowed us to better understand the coexistence of the two G-quadruplex conformations in equilibrium and how different structural elements conspire to favor a particular form. | |||
Coexistence of two distinct G-quadruplex conformations in the hTERT promoter.,Lim KW, Lacroix L, Yue DJ, Lim JK, Lim JM, Phan AT J Am Chem Soc. 2010 Sep 8;132(35):12331-42. PMID:20704263<ref>PMID:20704263</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
== | |||
< | |||
[[Category: Lacroix, L.]] | [[Category: Lacroix, L.]] | ||
[[Category: Lim, J K.C.]] | [[Category: Lim, J K.C.]] | ||
Revision as of 11:30, 20 October 2014
Structure of a (3+1) G-quadruplex formed by hTERT promoter sequence
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