2xky: Difference between revisions
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[[ | ==Single particle analysis of Kir2.1NC_4 in negative stain== | ||
<StructureSection load='2xky' size='340' side='right' caption='[[2xky]], [[Resolution|resolution]] 17.20Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2xky]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XKY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XKY FirstGlance]. <br> | |||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2gix|2gix]], [[1u4f|1u4f]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xky FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xky OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xky RCSB], [http://www.ebi.ac.uk/pdbsum/2xky PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The interaction of the extra-membranous domain of tetrameric inwardly rectifying Kir2.1 ion channels (Kir2.1NC(4)) with the membrane associated guanylate kinase protein PSD-95 has been studied using Transmission Electron Microscopy in negative stain. Three types of complexes were observed in electron micrographs corresponding to a 1:1 complex, a large self-enclosed tetrad complex and extended chains of linked channel domains. Using models derived from small angle X-ray scattering experiments in which high resolution structures from X-ray crystallographic and Nuclear Magnetic Resonance studies are positioned, the envelopes from single particle analysis can be resolved as a Kir2.1NC(4):PSD-95 complex and a tetrad of this unit (Kir2.1NC(4):PSD-95)(4). The tetrad complex shows the close association of the Kir2.1 cytoplasmic domains and the influence of PSD-95 mediated self-assembly on the clustering of these channels. | |||
Self-directed assembly and clustering of the cytoplasmic domains of inwardly rectifying Kir2.1 potassium channels on association with PSD-95.,Fomina S, Howard TD, Sleator OK, Golovanova M, O'Ryan L, Leyland ML, Grossmann JG, Collins RF, Prince SM Biochim Biophys Acta. 2011 Jul 5;1808(10):2374-2389. PMID:21756874<ref>PMID:21756874</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
==See Also== | ==See Also== | ||
*[[Potassium Channel|Potassium Channel]] | *[[Potassium Channel|Potassium Channel]] | ||
== References == | |||
== | <references/> | ||
< | __TOC__ | ||
</StructureSection> | |||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Collins, R F.]] | [[Category: Collins, R F.]] | ||
Revision as of 13:16, 22 October 2014
Single particle analysis of Kir2.1NC_4 in negative stain
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