2y9e: Difference between revisions
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[[ | ==STRUCTURAL BASIS FOR THE ALLOSTERIC INTERFERENCE OF MYOSIN FUNCTION BY MUTANTS G680A AND G680V OF DICTYOSTELIUM MYOSIN-2== | ||
<StructureSection load='2y9e' size='340' side='right' caption='[[2y9e]], [[Resolution|resolution]] 3.40Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2y9e]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y9E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2Y9E FirstGlance]. <br> | |||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1mne|1mne]], [[1d1c|1d1c]], [[1w9l|1w9l]], [[1mmn|1mmn]], [[1vom|1vom]], [[2jhr|2jhr]], [[2aka|2aka]], [[2y8i|2y8i]], [[1w9j|1w9j]], [[2x9h|2x9h]], [[1fmv|1fmv]], [[1mma|1mma]], [[2xo8|2xo8]], [[2y0r|2y0r]], [[1mmg|1mmg]], [[1w9i|1w9i]], [[1w9k|1w9k]], [[1mnd|1mnd]], [[1jx2|1jx2]], [[1q5g|1q5g]], [[1d0y|1d0y]], [[2jj9|2jj9]], [[2xel|2xel]], [[1lvk|1lvk]], [[1jwy|1jwy]], [[1yv3|1yv3]], [[1fmw|1fmw]], [[1d0x|1d0x]], [[1d1b|1d1b]], [[1g8x|1g8x]], [[1d1a|1d1a]], [[1d0z|1d0z]], [[1mmd|1mmd]]</td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Myosin_ATPase Myosin ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.1 3.6.4.1] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2y9e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y9e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2y9e RCSB], [http://www.ebi.ac.uk/pdbsum/2y9e PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The cold-sensitive single-residue mutation of glycine 680 in the reactive thiol region of Dictyostelium discoideum myosin-2 or the corresponding conserved glycine in other myosin isoforms has been reported to interfere with motor function. Here we present the x-ray structures of myosin motor domain mutants G680A in the absence and presence of nucleotide as well as the apo structure of mutant G680V. Our results show that the Gly-680 mutations lead to uncoupling of the reactive thiol region from the surrounding structural elements. Structural and functional data indicate that the mutations induce the preferential population of a state that resembles the ADP-bound state. Moreover, the Gly-680 mutants display greatly reduced dynamic properties, which appear to be related to the recovery of myosin motor function at elevated temperatures. | |||
Structural Basis for the Allosteric Interference of Myosin Function by Reactive Thiol Region Mutations G680A and G680V.,Preller M, Bauer S, Adamek N, Fujita-Becker S, Fedorov R, Geeves MA, Manstein DJ J Biol Chem. 2011 Oct 7;286(40):35051-60. Epub 2011 Aug 13. PMID:21841195<ref>PMID:21841195</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
== | |||
< | |||
[[Category: Dictyostelium discoideum]] | [[Category: Dictyostelium discoideum]] | ||
[[Category: Myosin ATPase]] | [[Category: Myosin ATPase]] | ||
